摘要
本文通过对中国亚洲玉米螟Ostrinia furnacalis(Guenée)17个地理种群的线粒体COⅡ基因进行序列分析,对中国亚洲玉米螟种群间的遗传分化程度和基因流水平进行了初步研究。在获得的413条序列样本中共发现了34个变异位点、35种单倍型。总体单倍型多样性指数Hd为0.811,种群内单倍型多样度在0.424～0.862范围内。17个种群间的平均基因流(Nm)为2.02。总群体的固定系数Fst为0.234。AMOVA分子方差分析结果表明中国玉米螟的遗传分化主要来自种群内部(76.45%)。各种群的TajimasD值中性检验符合中性突变,说明中国亚洲玉米螟在历史上没有出现群体扩张,群体大小稳定。各地理种群的遗传距离与地理距离间不具有显著的相关性。各地理种群中的单倍型在系统发生树上散布在不同的分布群中,缺乏明显的地理分布格局。
To investigate the genetic differentiation and gene flow among different populations of the Asian corn borer,Ostrinia furnacalis （Guenée）,in China,we analyzed COⅡ sequences of mitochondrial DNA in 17 geographical populations.In the total 413 COⅡ gene sequences obtained,34 variable sites and 35 haplotypes were observed.The mean haplotype diversity of all populations was 0.811,whereas it varied from 0.424 to 0.862 within population.The average gene flow （Nm） among the 17 populations was 2.02.The overall Fst value was 0.234.Molecular variance （AMOVA） analysis indicated that a high proportion of the total genetic variance was attributable to variations within populations （76.45%）.The Tajima＇s D value neutrality test results suggest that for the Asian corn borer,there might be no population expansion,so their population sizes were steady.The genetic distance was not significantly correlated with the geographical distance between populations.The phylogenetic tree revealed no obvious geographical pattern for the prevalence and distribution of the haplotypes.
出处
《昆虫学报》
CAS
CSCD
北大核心
2010年第10期1135-1143,共9页
Acta Entomologica Sinica
基金
转基因生物新品种培育科技重大专项(2008ZX08011-003)
国家"十一五"支撑计划课题(2006BAD08A06)
国家玉米产业技术体系资金资助
关键词
亚洲玉米螟
地理种群
线粒体COⅡ基因
单倍型
遗传分化
基因流
Ostrinia furnacalis
geographic population
mitochondrial COⅡ gene
haplotype
genetic differentiation
gene flow
