摘要
生物传感器在临床医学、环境和食品工业等方面都有重要的用途［１］，且由于其具有体积小、精度高、灵敏度好和便于现场测定等优点，已成为当前研究的热点课题之一．其中研究得较多的是氧化酶电流型生物传感器，特别是介体型，由于其响应快，灵敏而倍受重视．现已报道的可...
A new method for preparation of amperometric glucose oxidase electrode based on enzyme immobilization by Ag sols has been investigated. GOD was simply mixed with Ag nanoparticles and cross linked with a polyvinyl butyl(PVB) medium by glutaradehyde and was coatted on electrode by dipping a platinum wire into the sol\|gel solution. In experiments, we discussed the effects of different kinds of silver gels on response of the electrode and the mechanism about it. For the optimum conditions, the current response of the enzyme electrode containing hydrophobic silver gels for 10 mmol/L glucose was enhanced to 18 200 nA as compared with the electrode without silver gels with 280 nA current response. In addition to the high sensitivity, the electrode responses rapidly, the 95% steady\|state response time is less than 20 s.\=
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1999年第4期634-636,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
银
超细颗粒
酶电极
生物传感器
葡萄糖氧化酶
Silver nanoparticle, Glucose oxidase, High sensitivity, Rapid response
