摘要
从某浓香型名酒厂百年老窖泥中分离到一株己酸高产菌系,可广泛应用于液态白酒和浓香型白酒生产。以芽孢菌数和己酸产量为指标,在单因素实验的基础上,采用正交试验,确定优化扩大培养条件为:乙醇2%、NaAc1%、K2HPO40.04%、酵母膏0.1%、初始pH7.0左右、接种量8%～10%、装液量95%左右、34～35℃培养9天,芽孢菌数可达4.5亿个/mL,已酸量达5g/L。
a high caproic acid yield strain was separated from a hundred year cellar in a famous Luzhou flavor liquor making factory,which could be widely used in the liquor produced by liquid fermentation and Luzhou flavor liquor production.Based on the single factor experiments,orthogonal test was carried out,using both the amount of spore bacteria and the yield of caproic acid as indexes.The optimum culture conditions were determined to be：ethanol 2%,NaAc 1%,K2HPO4 0.04%,initial pH 7.0,inoculum size of 8%-10%,medium volume of 95%,34-35 ℃ for nine days.The spore bacteria numbers were up to 4.5 ×109cell/mLand the yield of caproic acid was 5 g/L.
出处
《酿酒》
CAS
2010年第6期18-21,共4页
Liquor Making
关键词
浓香型白酒
己酸菌
培养条件
优化
luzhou-flavor liquor
already acid bacteria
cultivating conditions
the optimization.
