摘要
目的应用SD大鼠建立慢性牙周炎（chronic periodontitis，CP）和慢性阻塞性肺疾病（chronic obstructive pulmonary disease，COPD）动物模型，探讨CP和COPD的相关机制。方法将40只sD大鼠按随机数字法分为4组：A组：正常对照组；B组：CP组；C组：COPD组；D组：CP＋COPD组，每组10只。各组按实验设计施加相应建模因素，于实验10周末处死动物。分别检查各组大鼠的牙周指标及肺功能状况，并观察牙周和肺部的组织病理学改变，酶联免疫吸附法检测血清中肿瘤坏死因子（TNF）-α水平，并行统计学分析。结果A、C组出血指数分别为（0．25±0．04）、（1．30±0．25），附着丧失分别为（0．43±0．02）、（0．51±0．02）mm，与A、C组相比，B、D组大鼠的出血指数[分别为（3．85±0．34）、（4．01±0．44）]、附着丧失[分别为（0．90±0．27）、（0．98±0．18）mm]均明显升高（P〈0．05）；与A组0．2s末用力呼气量与用力肺活量比值（forced expiratory volumein 0．2 second toforcedvitalcapitalratio，FEV0.2／FVC值）（65．34±2．63）相比，B、C、D组大鼠FEV0.2／FVC值[分别为（57．49±6．77）、（58．10±2．40）、（49．53±2．86）]明显降低，表明动物模型建造成功。其中D组肺功能与病理学改变均较c组更大，各实验组与正常对照组TNF—α比较差异均有统计学意义（P〈0．05），D组TNF-α【含量[（23．422±2．815）μg／L]升高最为明显。结论在CP和COPD相关关系大鼠动物模型中，CP可能对COPD具有促进和诱发作用。
Objective To establish a model of chronic periodontitis（CP） accompanied with chronic obstructive pulmonary disease （COPD） in SD rats and investigate the relationship between chronic periodontitis and COPD. Methods Equal gender SD rats were randomly divided into 4 groups, A： control group, B： CP group, C： COPD group, D： COPD with CP group （n =10, respectively）. Each group was subjected to its predesigned intervention to establish a specific disease model. After 10 weeks, animals were sacrificed. The level of alveolar bone loss, lung function measurement, and the histopathologieal changes of periodontal and lung tissues were examined. The serum tumor necrosis factor （TNF）-α level was detected by enzymelinked immunoabsorbent assay kits. Results Bleeding index （BI） levels of group A and C were （0. 25 ±0. 04 ） and （ 1.30 ± 0. 25 ）, respectively. Attachment loss was （0.43 ± 0.02 ） and （ 0. 51 ± 0. 02） mm. BI levels in group B and D were significantly higher than those in group A and C. Forced expiratory volume in O. 2 second to forced vital capital ratio（FEV0.2/FVC） values in group B, C and D were significantly lower than that in group A. Pulmonary function were worse in group D than that in group C（ P 〈 0. 05 ）. The levels of serum TNF-α, a sensitive indicator of both diseases, were increased in all test groups compared with the control, and increased most in group D. Conclusions The chronic periodontitis and chronic obstructive pulmonary disease model was established in SD rat. The chronic periodontitis may be a risk factor for promoting and inducing COPD.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2010年第11期668-672,共5页
Chinese Journal of Stomatology
基金
国家自然科学基金（30872878）
北京市自然科学基金（7093120）
关键词
牙周炎
肺疾病
慢性阻塞性
模型
动物
Periodontitis
Pulmonary disease, chronic obstructive
Models,animal
