摘要
目的:检测细胞周期抑制因子p57kip2在肝癌(HCC)中的表达水平,探讨它与各种临床病理因素、增殖凋亡的关系及其预后价值。方法:用免疫组织化学方法检测40例肝癌组织、40例癌旁组织和15例正常肝脏组织中p57kip2的表达水平;用DNA缺口末端标记技术检测肝癌组织中细胞凋亡水平。结果:p57kip2在肝癌组织中的阳性率42.5%,显著低于癌旁肝组织(72.5%)和正常肝脏组织(93.3%)(P<0.05),p57kip2的缺失表达与晚期肝癌和肝外转移有关(P<0.05)。HCC组织中存在增殖凋亡异常(P<0.05)。p57kip2的阳性表达与HCC组织低增殖有关(P<0.05),与细胞凋亡无关(P>0.05)。TNM分期、肝外转移,AFP水平与p57kip2蛋白表达与HCC预后有关(P<0.05),且p57kip2和临床分期是HCC的独立预后因素(P<0.05)。结论:p57kip2参与了肝癌的发生发展,可用于预测晚期肝癌预后。
Objective：To evulate the expression level of p57^kip and relationships of p57^kip and clinicopathological factors,cell proliferation and apoptosis in the genesis and progression of HCC and the prognostic importance of p57^kip in HCC.Methods：We evaluated p57^kip and PCNA by immunohistochemistry and apoptosis indices by TUNEL in 40 HCC tissues and compared to 40 adjacent non-cancerous tissues and 15 normal liver tissues.Results：Expression of p57^kip in HCC（42.5%） was significantly lower than that in adjacent non-cancerous tissues（72.5%） and normal liver tissues（93.3%）（P 0.05）.The absence of p57^kip was associated with advanced HCC and extrahepatic metastasis（P 0.05）.Moreover,the dysregulation between proliferation and apoptosis were observed in HCC tissues（P 0.05）.The positive expression of p57^kip was related to low proliferation（P 0.05）,but there was no relationship between the expression of p57^kip and apoptosis.In univariate analysis,TNM stage,extrahepatic metastasis,AFP level,p57^kip had prognostic significance in HCC（P 0.05）.The abnormal expression of p57^kip and TNM stage were independent prognostic factors of HCC in multivariate analysis（P 0.05）.Conclusion：p57^kip participates the occurrence and progression of HCC,and its expression status is a potential candidate prognostic marker of advanced HCC.
出处
《现代肿瘤医学》
CAS
2010年第12期2439-2443,共5页
Journal of Modern Oncology
关键词
P57KIP2
肝细胞肝癌
临床病理因素
细胞增殖凋亡
预后
p57^kip2
hepatocellular carcinoma
clinicopathological factors
proliferation and apoptosis
prognosis
