摘要
目的探讨趋化因子受体CX3CR1基因多态性与北方汉族人群脑梗死发病相互关系。方法收集120例脑梗死患者和102例年龄、性别相匹配的健康对照者抗凝血标本,采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)技术检测趋化因子受体CX3CR1基因型,用以研究趋化因子受体CX3CR1 T280M及V249I基因变异在两组中的差异。结果CX3CR1-T280M及CX3CR1-V249I的基因多态性在脑梗死组和对照组中的差异有显著性,经调整后的OR值分别为0.48(95%CI:0.28～0.83,P<0.05)和0.49(95%CI:0.26～0.91,P<0.05)。I249等位基因频率在两组中也存在着显著性差异,经调整后的OR值为0.54(95%CI:0.35～0.84,P<0.05)。I249等位基因能显著减少脑梗死的发病率。结论等位基因CX3CR1-I249能降低脑梗死的发病率,V249I的基因多态性是脑梗死的独立保护因素。
【Objective】We investigated the risk of cerebral infarction （CI） with fractalkine receptor CX3CR1 polymorphisms. 【Methods】We investigated the T280M and V249I mutations in the CX3CR1 gene in 120 Chinese patients with cerebral infarction （CI） and 102 age-and sex-matched healthy controls. Polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP） were used for genotyping. 【Results】There was significant difference in both polymorphisms between patients with CI and controls,the adjusted odds ratio respectively was 0.48 （95% CI：0.28～0.83,P〈0.05） and 0.49 （95% CI：0.26～0.91,P〈0.05）. There was significant difference in frequencies of allele I249 between cerbral infarction and control groups. The adjusted odds ratio was 0.54 （95% CI：0.35 ～0.84,P〈0.05）. CX3CR1 I249 was associated with a markedly reduced risk of cerebral infarction. 【Conclusion】The results show that CX3CR1 I249 is an independent genetic risk factor for cerebral infarction and that CX3CR1 may be involved in the pathogenesis of athero sclerotic disease.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2010年第1期4-8,共5页
China Journal of Modern Medicine
基金
辽宁省自然科学资金资助(No:20082179)
