摘要
以酱油渣干粉为原料,对大豆异黄酮提取条件和纯化方法进行研究.采用实验室模拟动态逆流提取酱油渣中异黄酮,并通过正交设计对提取条件进行了优化,确定了最佳提取条件为体积分数为80%乙醇、提取前的浸泡时间12 h、料液比1∶10,总异黄酮提取率可达0.35%,粗提物的纯度为2.01%.选取3种极性不同的大孔树脂填装制备柱,并利用中低压色谱对大豆异黄酮样品进行纯化.结果表明,NKA9大孔树脂对总大豆异黄酮的纯化效果最好,在体积分数为60%乙醇洗脱时,产品纯度可达到32%.该方法能在短时间内对大豆异黄酮样品进行大量的纯化,满足大豆异黄酮产品生产的需要.
Using the powder of soy sauce residue as raw material,the extraction and purification methods of soy isoflavone were investigated.An orthogonal design for the dynamic countercurrent extraction of isoflavones from soy sauce residue were tested at laboratory level.The optimized conditions were determined to be with 80% ethanol,soaked with a material to liquid ratio of 1∶10 for 12 h before extraction.The extraction rate of total isoflavones was 0.35% with a content of 2.01% in the crude extracts.Using the low pressure chromatography and three kinds of macroporous resins,the purification of soybean isoflavones in the extracts was performed.A isoflavone product with a purity of 32% was obtained through NKA9 macroporous resin colomn chromatography.The method can be used for treatment of large numbers of samples within a short period of time,and the product can meet market needs of soy isoflavone.
出处
《华南师范大学学报（自然科学版）》
CAS
北大核心
2010年第4期80-86,共7页
Journal of South China Normal University(Natural Science Edition)
基金
广东省科技计划项目(2008B060411031)
关键词
酱油渣
大豆异黄酮
动态逆流提取
中低压色谱
soy sauce residue
soy isoflavones
dynamic countercurrent extraction
medium-pressure liquid chromatography
