摘要
[目的]研究一种不使用切片机的石蜡制片方法,同时提出一种对植物解剖学数码照片进行光学信息解析的方法。[方法]将材料包埋成蜡块,在解剖镜下对材料蜡块进行切片,将切下的薄蜡片卷转移到载玻片上加热,石蜡融化后便将切片展平,再经常规或简化的染色制片程序完成封片。对植物解剖学数码照片处理时,先将其拷入word文档,在文档中获得2份相同的照片,用鼠标选定(刷黑)其中一张,使其成为原照片的"负片",然后复制整个屏幕,将其粘贴到文档中,经剪切等操作后获得处理后的照片。[结果]获得了比较理想的植物解剖学制片。龙爪柳叶片结构的数码照片经过处理后,具有一定的立体感,其角质层和细胞壁等结构及细胞的识别更加容易。[结论]该方法成本低、效率较高,可供初学者或无切片机时使用。对解剖学数码照片进行光学信息解析,能获得普通数码照片无法显示的一些结构信息,显示出光学信息解析的潜力与前景。
[Objective]The research aimed to study a paraffin method without using the microtome,and also introduced an analysis method of the plant-anatomical digital photographs for optical information.[Method]The plant material softened or not was embedded in paraffin according to the paraffin method.Cut the thin paraffin sections from the paraffin block with a sharp two-sided blade under anatomical lens.The thin material sections rolled up when they were cut off.Took the section rolls to a slide,and then heated them to melt the paraffin section roll.When the paraffin melted,the sections of plant material were rolled out.After the common or simplified procedures staining and mounting,the preparations were finished.When a anatomical digital photograph was processed,copy it into the word file and get two copies of the original photograph.Selected a copy to make it to be a negative photograph,and then press the key ＂Press Screen＂ to copy the screen frame.After copied it into the word file,cut the unnecessary parts and used other operations,and got the processed photograph.[Result]Using this method,the anatomical preparation for research can be gotten.The analyzed digital photograph of the leaf structure of Salix matsudana f.tortuosa has some a three-dimensional effect,and the different leaf structures and cells,e.g.cuticle,cell wall,protoplast,vein,etc.can be identified easily.[Conclusion]The paraffin method without using the microtome has advantage of low-coast and higher-efficiency,which could be applied by the beginner or in the time without a microtome to be used.The analysis of the plant-anatomical digital photographs can acquired more structural information than the original digital photographs,which shows the potentiality and prospects of the optical information analysis of the microscopic imagery and its digital photograph.
出处
《安徽农业科学》
CAS
北大核心
2010年第32期17996-17998,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(30770124)
关键词
石蜡切片法
数码照片
光学信息
解析
Paraffin method
Digital photograph
Optical information
Analysis
