摘要
目的 通过对接受ICSI治疗的不育患者进行精子形态分析和ICSI结果参数的统计分析,研究精子形态对于ICSI结果参数的影响.方法 采用改良巴氏染色法进行精子形态学分析,对精子正常形态率和ICSI的受精率、卵裂率、优质胚胎率、胚胎移植率、临床妊娠率等结果参数进行相关性分析、分组秩和检验和卡方检验.结果 ①精子正常形态率与受精率无相关性（r=0.02,P〉0.05）、与卵裂率无相关性（r=-0.17,P〉0.05）、与优质胚胎率无相关性（r=-0.10,P〉0.05）、与胚胎移植率无相关性（r=0.05,P〉0.05）.②以精子正常形态率4%为界分为两组,精子正常形态率〈4%（n=24）组,受精率（80.29%）、卵裂率（98.75%）、优质胚胎率（43.54%）、胚胎移植率（31.30%）.精子正常形态率≥4%（n=32）组,受精率（79.68%）、卵裂率（94.52%）、优质胚胎率（41.07%）、胚胎移植率（37.8%）,两组之间的秩和检验差异无显著性（P〉0.05）.两组的临床妊娠率的卡方检验差异无显著性（P〉0.05）.结论 精子形态不影响ICSI的受精率、卵裂率、优质胚胎率、胚胎移植率、临床妊娠率等结果参数.
Objective To explore the influence of sperm morphology to some consequence parameter of intracytoplasmic sperm injection （ICSI）, by sperm morphology analysis and statistical analysis to infertility patients in ICSI therapy. Methods We accounted the rate of morphologically normal sperm by Kruger＇ s strict criteria , and analyzed the normal sperm morphology rate and the rates of fertilization, cleavage, embryo quality, embryo transfer and clinical pregnancy by correlation analysis,rank-sum test and chi square test. Reaults The normal sperm morphology rate did not show any correlation with the rates of fertilization, cleavage, embryo quality and embryo transfer;, no statistic differences were found in the rates of fertilization, cleavage, embryo quality, embryo transfer and clinical pregnancy between the normal sperm morphology rate 〈 4% and ≥ 4% groups （P〉0.05）. Conclusion Sperm morphology does not influence some consequence parameter of ICSI, such as the rates of fertilization,cleavage, embryo quality, embryo transfer and clinical pregnancy.
出处
《国际医药卫生导报》
2010年第24期2959-2962,共4页
International Medicine and Health Guidance News
基金
广东省江门市科技计划项目（2008-042）
关键词
精子形态
精子正常形态率
ICSI
受精率
临床妊娠率
Sperm morphology
Normal sperm morphology rate
ICSI
Fertilization rate
Clinical pregnancy rate
