摘要
为研究肝癌ＭＤＲ机制，采用５种抗癌药物通过不同的诱导方式建立一组人肝癌ＭＤＲ细胞模型。对其耐药机理的研究结果表明，ＳＭＭＣ７７２１／ＤＯＸ亚系由ｍｄｒ１基因介导耐药，ＳＭＭＣ７７２１／ＶＣＲ亚系由ＭＲＰ基因介导耐药，ＳＭＭＣ７７２１／ＣＢＰ亚系由ＬＲＰ基因介导耐药，ＳＭＭＣ７７２１／ＶＰ１６亚系由ＴｏｐｏⅡα基因介导耐药，ＳＭＭＣ７７２１／ＭＭＣ亚系由ＧＳＴｐ１基因介导耐药，多重ＭＤＲ亚系ＳＭＭＣ７７２１／Ｍ的耐药涉及ｍｄｒ１、ＭＲＰ、ＬＲＰ、ＴｏｐｏⅡα和ＧＳＴｐ１基因。
To study multidrug resistance(MDR)mechanism of hepatocellular Carcinoma(HCC),By using five kinds of chemotheraputic agents,a group of MDR cell sublines in HCC cell were successfully established.Observation on models drug resistance mechanism revealed that drug resistance in SMMC7721/DOX subline was mediated by mdr1 gene;similarly was that the SMMC7721/VCR subline mediated by MRP gene;SMMC7721/CBP subline by LRP gene;SMMC7721/VP16 subline by Topogene;and SMMC7721/MMC subline by GSTP1gene.According to the complex-MDR subline SMMC7721/M,drug resistance was mediated by mdr1,MRP,LRP,Topo and GSTp1genes collectively.
出处
《肝胆外科杂志》
1999年第2期153-155,共3页
Journal of Hepatobiliary Surgery
基金
福建省重点攻关项目基金
关键词
多药耐药性
细胞模型
肝癌
CarcinomaHepatocellularMultidrug resistanceCell sublines
