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锌对前列腺癌22RV1细胞的增殖及ZIP4mRNA表达的影响 被引量：1

Effect of Zinc on the Proliferation of Prostate Cancer Cell Line 22RV1 and the Expression of ZIP4 mRNA
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摘要 目的探讨锌对前列腺癌22RV1细胞的增殖及对ZIP4 mRNA表达的影响。方法采用倒置相差显微镜观察并采用MTT法检测不同浓度锌(0.5、5、50、100μmol/L)对前列腺癌22RV1细胞的形态及增殖活性的影响,实时定量RT-PCR法检测不同浓度锌在不同时间点对ZIP4 mRNA表达的影响。结果在锌浓度为0.5μmol/L时,细胞皱缩,形态发生明显变化,增殖活性下降,与对照组相比差异具有统计学意义(P<0.01),其余3组未见明显变化(P>0.05)。随着锌作用时间的延长,ZIP4mRNA表达量下降,36 h达最低值之后保持恒定;当锌浓度为0.5μmol/L时,ZIP4 mRNA的表达量变化与对照组相比无统计学差异(P>0.05),其余各浓度组变化均有统计学差异(P<0.01)。结论锌在一定浓度水平上可以抑制前列腺癌22RV1细胞的增殖活性,且对ZIP4 mRNA的表达具有时间和剂量依赖性。 Objective To explore the effect of zinc on the proliferation of prostate cancer cell line 22RV1 and the expression of ZIP4 mRNA.Methods The morphology of 22RV1 cells exposed to different concentrations of zinc was observed under phase contrast microscope,and the proliferative activity of 22RV1 cells was detected by MTT assay.The effect of different concentrations of zinc on the expression of ZIP4 mRNA was determined by real-time quantitative polymerase chain reaction at different time points.Results In 22RV1 cells exposed to zinc of 0.5 μmol/L,obvious morphological changes were found,and the proliferative activity significantly decreased compared with control group（P 0.01）.No signficant difference in the proliferative activity was found between 22RV1 cells exposed to zinc of 5,50,and 100 μmol/L and control group（P 0.05）.The expression of ZIP4 mRNA decreased with the duration of zinc exposure and reached the lowest level after 36 hours,and then the level of ZIP4 mRNA maintained.Compared with the control group,the expression of ZIP4 mRNA significantly decreased in 22RV1 cells exposed to zinc of 5,50,and 100 μmol/L,except for the cells exposed to zinc of 0.5 μmol/L.Conclusion Within a certain range of concentration,zinc can inhibit the proliferation of prostate cancer cell line 22RV1,and has a time-and dose-dependent effect on the expression of ZIP4 mRNA.
作者 陈启光 马东蔚 张哲 单广夷 孔垂泽
机构地区 中国医科大学附属第一医院泌尿外科 中国医科大学附属第一医院内分泌科
出处 《中国医科大学学报》 CAS CSCD 北大核心 2010年第11期908-911,共4页 Journal of China Medical University
基金 辽宁省科技计划资助项目(2007225002-1 2007216010)
关键词 锌 前列腺癌 22RV1 增殖 ZIP4 zinc prostate cancer 22RV1 proliferation ZIP4
分类号 R737.25 [医药卫生—肿瘤]
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参考文献13

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同被引文献11

  • 1Mao X, Kim BE, Wang F, et al. A histidine-rich cluster mediates the ubiquitination and degradation of the human zinc transporter, hZIP4, and protects against zinc cytotoxicity [J]. J Biol Chem, 2007, 282(10) : 6992-7000.
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中国医科大学学报
2010年 第11期

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