摘要
目的建立鳜致病性嗜水气单胞菌灭活疫苗原液的生产工艺。方法分别采用摇瓶和5L发酵罐于28℃培养嗜水气单胞菌GYK1株,测定不同时间菌液的A650值;培养菌液添加不同浓度的甲醛液,检测不同时间的灭活效果;依次采用摇瓶、种子罐、发酵罐培养,甲醛灭活,制备灭活疫苗原液,并进行安全性和效力试验。结果摇瓶培养时,培养菌液18h进入生长稳定期,24h左右达高峰;用5L发酵罐培养时,培养菌液8h即进入生长稳定期,10h左右达高峰;0.30%的甲醛,37℃,24h可完全灭活菌液,且甲醛残留量较低;制备的灭活疫苗原液安全性良好,效力合格。结论初步建立了鳜致病性嗜水气单胞菌灭活疫苗原液的生产工艺。
Objective To develop a production procedure for the bulk of inactivated mandarinfish pathogenic Aeromonas hydrophila vaccine.Methods Aeromonas hydrophila GYK1 strain was cultured in shake-flask and 5 L fermenter for various hours and determined for A650.The bacterial culture was inactivated with formaldehyde solution at various concentrations for various hours,and the inactivation effect was evaluated.The strain was cultured in shake-flask,seed tank and fermenter in turn,then inactivated with formaldehyde to prepare the bulk of inactivated vaccine which was tested for safety and potency.Results The growth of strain reached a stable stage 18 h and a peak value 24 h after culture in shake-flask.However,in 5 L fermenter,the growth of strain reached a stable stage 8 h and a peak value about 10 h after culture.The bacterial liquid was completely inactivated with 0.30% formaldehyde at 37℃ for 24 h,and the residual formaldehyde was low.The prepared bulk of inactivated vaccine showed high safety and was qualified in potency test.Conclusion A production procedure for the bulk of inactivated mandarinfish pathogenic Aeromonas hydrophila vaccine was preliminarily developed.
出处
《中国生物制品学杂志》
CAS
CSCD
2010年第11期1222-1225,共4页
Chinese Journal of Biologicals
基金
农业公益性行业科研专项(200803013)
农业科技成果转化资金项目(2008GB23260393)
关键词
嗜水气单胞菌
疫苗
灭活
生产工艺
Aeromonas hydrophila
Vaccine
inactivated
Production procedure
