摘要
旨在探讨丹参对链脲佐菌素(STZ)糖尿病模型大鼠的血糖及脾、肾组织结构的影响,为临床预防和治疗糖尿病并发症提供参考。60mg·kg-1腹腔注射链脲佐菌素制备糖尿病大鼠模型,将符合糖尿病诊断标准的大鼠,随机分为模型对照组、丹参治疗组,各15只。另外以20只正常大鼠设立正常对照组。丹参治疗组灌胃丹参水煎液5g·kg-1·d-1,模型对照组和正常对照组灌胃相当剂量的蒸馏水,试验期为28d。给药后的第7、14、21、28天眶后静脉丛采血,离心制备血清测血糖。试验结束后剖杀大鼠,取脾、肾组织,Bouin′s液固定,常规石蜡切片,HE染色,观察切片的组织结构。试验第28天,丹参治疗组的血糖极显著低于模型对照组(P<0.01),极显著高于正常对照组(P<0.01);模型对照组脾小结数量较少,较难见到生发中心,动脉周围淋巴鞘较薄;肾小球体积增大,血管球扩张,肾小囊囊腔变小,肾小管腔里充有不等量蛋白渗出物,肾小管上皮细胞脱落。而丹参治疗组脾小结界限较清晰,可以见到生发中心,动脉周围淋巴鞘较薄,淋巴细胞排列相对较紧密;肾小球、肾小管排列规则,囊腔隙较正常,肾小管结构清晰、上皮细胞结构较正常,但管腔里内还有少量均质红染的蛋白渗出物。丹参在一定程度能够改善糖尿病大鼠糖代谢的异常,丹参对模型大鼠脾、肾组织结构病变有所缓解。
To study the effect of Danshen Root on the glucose,the structure of spleen and kidney in diabetic rats induced by STZ.And the diabetes model was established by Streptozotocin injected 60 mg·kg-1.The SD rats were randomly divided into two groups as diabetes group and Danshen treated group,15 in each group.There were 20 rats in Normal control group.Danshen treated group were orally administered by Danshen Root boiled liquid（5 g·kg-1·d-1）,and the diabetes group,normal control group were administered by the similar dosage of distilled water.The period of experiment was 28 days.At the 7th,14th,21th and 28th day,glucose was measured in serum that is prepared by taking blood through orbital venous plexus and centrifuging.At the end of the experiment,histological section of all rats′ spleen and kidney were made and observed after Bouin liquid fixing and HE staining.Glucose of Danshen treated group were significantly lower than that of the model control group（P0.01） and higher than that of the normal control group（P0.01）.For the model control group,the following results of histological observation showed that there were less splenic cords,invisible germinal centers and thinner periarterial lymphatic sheath,larger renal glomerulus,smaller renal capsule,protein exudates and exfoliative epithelial cells in renal tubule.For the Danshen treated group,the observation results showed that splenic cords with clear demarcation,visible germinal centers and thicker periarterial lymphatic sheath,closely aligned lymphocytes,normally aligned renal glomerulus,renal capsule and renal tubule,normal epithelial cells but a small quantity of homogeneously red-stained protein exudates.The results showed that Danshen Root could improve the abnormal glycometabolism of diabetic rats and help prevent the histological change of the spleen and kidney for diabetes model rats.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第11期1467-1471,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
安徽科技学院引进人才专项基金资助项目(ZRC2007139)
关键词
糖尿病
链脲佐菌素
丹参
血糖
脾
肾
diabetic mellitus
STZ
Danshen Root
glucose
spleen
kidney
