摘要
目的观察慢性荨麻疹血清对嗜碱粒细胞CD203c表达的影响,以建立流式细胞术检测慢性荨麻疹血清中自身抗体的方法。方法以CRTH2-FITC、CD203c PE和CD3Per-CP 3种抗体标记白细胞,建立嗜碱粒细胞活化试验,用流式细胞仪测定细胞活化及荧光强度。结果自身血清皮肤试验(ASST)阳性组CD203c阳性细胞的百分率为91.24±6.22,与ASST阴性组(3.33±1.24)及健康对照组(3.05±1.07)比较具有明显差异(P<0.01);ASST阴性组与健康对照组比较无显著差异。ASST阳性组CD203c平均荧光强度(MFI)为58.2±12.55,显著高于ASST阴性组(3.70±1.32,P<0.01)和健康对照组(3.20±1.29,P<0.01),ASST阴性组与健康对照组的MFI比较无显著差异。结论用CRTH2-FITC、CD203c-PE和CD3Per-CP 3种抗体,可以观察嗜碱粒细胞表面的CD203c表达程度,以判断荨麻疹血清对嗜碱粒细胞的活化。
Objective To evaluate the effects of chronic urticaria sera on CD203c expressions on basophils so as to construct ways to detect autoantibodies in chronic urticaria sera by flow cytometry.Methods Using CRTH2-FITC、CD203c-PE and CD3Per-CP fluorescent monoclonal antibodies,basophil activation test were constructed to assay cell activations and fluorescent intensities.Results Incubation of donor basophils with sera from patients with chronic urticaria and positive autologous serum skin test（ASST）demonstrated significant increasing positive basophil percent,91.24±6.22 vs 3.33±1.24,P〈0.01;and upregulation of CD203c expression,the mean fluorescent intensities（MFI）58.2±12.55 vs 3.70±1.32,P〈0.01.There are no statistically differences between the ASST negative group and the health group.Conclusion The CD203c expressing on basophils can effectively be observed by CRTH2-FITC,CD203c-PE and CD3Per-CP fluorescent monoclonal antibodies and making gates three times on flow cytometry,so that basophil activations by chronic urticaria sera can be evaluated.
出处
《重庆医学》
CAS
CSCD
北大核心
2010年第23期3200-3201,共2页
Chongqing medicine
基金
国家自然科学基金资助项目(30972647)
第三军医大学科研基金资助项目(2008XG179)
关键词
慢性荨麻疹
自身抗体
流式细胞术
chroncic urticaria
autoantibodies
flowcytometrics
