摘要
目的观察小于胎龄）L（SGA）大鼠胰岛素敏感指数和ghrelin水平的变化及相关关系；探讨ghrelin在SGA导致胰岛素抵抗过程中的作用和意义。方法采用SD大鼠母鼠妊娠期饥饿法建立SGA大鼠模型，按生后第4周身长和（或）体质量的差异分为有生长追赶组（sl组，n=26）、无生长追赶组（S2组，n=31）；以自南饮水及自由进食母鼠娩出之雄性鼠仔为正常对照组（C组，n=27）。各组大鼠4周龄时测定体质量及血ghrelin水平，12周龄测定体质量及血ghrelin、空腹血糖、空腹胰岛素并计算胰岛素敏感指数，分析各指标之间的相关性。结果12周龄SGA大鼠胰岛素敏感指数较C组下降（S1组：2．00±0．58，S2组：2．23±0．58比C组：3．17±0．54，均P〈0．05）；血ghrelin水平较C组下降，但差异无统计学意义[S1组：（1．357±0．548）μg／L，S2组：（1．428±0．714）μg／L比C组：（1．843±0．459）μg/L，均P〉0．05]。12周龄大鼠血ghrelin水平与空腹血胰岛素水平呈负相关（r=-0．836，P〈0．01），而与胰岛素敏感指数呈正相关（r=0．810，P〈0．01）。结论成年SGA大鼠血ghrelin水平降低与胰岛素抵抗有关，低ghrelin水平可能是胰岛素抵抗的结果。
Objective To observe the change of insulin sensitivity index （ISI） and ghrelin level, to examine their correlation and to explore the role and significance of ghrelin in insulin resistance （IR） in small for gestational age （SGA） rats. Methods The SGA models were established by starvation method in pregnant SD rats. Experimental rats were grouped by body length and/or body weight 4 weeks after birth. Experiment grouping： （ 1 ）SGA group with growth catch-up（Group S1, n=26） ; （2）SGA group without growth catch-up （Group S2, n=31 ） ; （3）Normal control group was composed by male rats whose mothers ate and drank freely during pregnancy （Group C, n=27 ）. Body weight and serum ghrelin level was checked 4 weeks after birth. Body weight, serum ghrelin, fasting blood glucose and fasting insulin were tested when the rats were 12 weeks old, and then ISI was calculated. Correlation of all the indexes was analyzed. Results The ISI of 12-week-old SGA rats was significantly decreased as compared to normal control （Group SI： 2.00＋ 0.58, Group $2：2.23~0.58 vs Group C： 3.17＋0.54, both P〈0.05）. Serum ghrelin level of SGA rats was decreased compared with Group C, but the disparity was not statistically significant [Group SI： （1.357 ± 0.548 ） μg/L, Group S2 ： （ 1.428±0.714） μg/L vs Group C ： （1.843±0.459） μg/L, both P〉0.05 ]. Ghrelin level in 12-week-old rats presented negative correlation with fasting insulin level in blood （r=-0.836, P〈0.01 ）, and positive correlation with ISI （r=0.810, P〈0.01 ）. Conclusion The decrease of serum ghrelin level is correlated with IR in adult SGA rats. Low serum level of ghrelin may be the result oflR.
出处
《中华生物医学工程杂志》
CAS
2010年第4期309-312,共4页
Chinese Journal of Biomedical Engineering
基金
基金项目：广东省科技厅科研基金（200780315100HD5）
