摘要
研究了稀硫酸介质中,槲皮素催化溴酸钾氧化吖啶红的荧光光谱。在优化实验条件下,确定了荧光强度改变值（ΔIF）与槲皮素质量浓度的关系,建立了催化动力学荧光法测定洋葱中槲皮素的新方法。该方法的线性范围为0.43-18.0μg.L^-1,检出限为0.13μg.L^-1。方法操作简便,具有较好的灵敏度,用于洋葱中槲皮素含量的测定,结果满意。
In sulphuric acid medium,the fluorescence signal of acridine red could be decreased by the oxidation of potassium bromate.In the presence of quercetin,the oxidation reaction was catalyzed,which could cause the fluorescence intensity of acridine red becoming weaker.And the reduced fluorenscence intensity was in proportion to the concentration of quercetin in a certain range.Based on this,a catalytic kinetic fluorescence method was established to detect quercetin in onion.Under the optimal conditions,the linear range for quercetin were between 0.43 and 18.0 μg·L-1 with a detection limit of 0.13 μg·L-1.The method was simple and sensitive,and was applied in the determination of quercetin in onion with satisfactory results.
出处
《分析测试学报》
CAS
CSCD
北大核心
2010年第12期1228-1230,1234,共4页
Journal of Instrumental Analysis
基金
湖南省教育厅课题(07C634)
关键词
槲皮素
催化
荧光法
吖啶红
quercetin
catalytic
fluorescence spectrophotometry
acridine red
