摘要
从白云边高温大曲中筛选得到1株产酸性蛋白酶活力较高的芽孢杆菌菌株,经VITEK-32微生物自动鉴定系统鉴定为地衣芽孢杆菌。通过对该菌株固体发酵条件的研究,发现其最适产酶条件为:最佳固体培养基为:固料∶水为1∶0.7,在固料中麸皮:黄豆粉为1∶0.8,pH5.0,培养温度为45℃,发酵时间为52h,接种量为7%,该菌株固体发酵产酶活力达到1420.2 U/g。
A acid protease producing strain was from high temperature Daqu was identified as Bacillus licheniformis with VITEK-32 microbe automatic appraisal system.According to the study of fermentation condition,we can find its fittest condition were as follows： the optimum composition of the solid substrate for fermentation was solid material∶water was 1∶ 0.7,bran∶bean flour was 1∶0.8 in the total solid material,initial pH is 5.0,culture temperature is 45℃,fermentation time is 52h,7% flask inoculums size.Under above conditions,the activity of acid-protease produced by the strain was 1420.2U/g.
出处
《食品与发酵科技》
CAS
2010年第6期32-35,共4页
Food and Fermentation Science & Technology
基金
国家自然科学基金项目"嗜热芽胞杆菌转座子突变体库的构建及产香机理研究"(31071594)资助
关键词
高温大曲
酸性蛋白酶
发酵条件
high temperature Daqu
acid-protease
fermentation condition
