摘要
目的:考察蓝萼香茶菜中蓝萼甲素提取工艺。方法:采用L9(34)正交试验法优选甲醇回流提取时间、提取次数、固/液比;采用HPLC测定蓝萼甲素含质量。结果:蓝萼香茶菜甲醇提取的最佳工艺条件为(A2B2C3),即提取时间为1.5h,回流提取2次,固/液比为1:15时,测得的蓝萼甲素含质量最高。结论:该法提取与测定蓝萼甲素含质量准确,数据可靠,适用于蓝萼香茶菜中蓝萼甲素含质量测定。
Objective：To study the extraction technique of glaucocalyxin A in Rabdosia japonica var.Glaucocalyx.Methords：Using L9（34） orthogonal experiment to optimize methanol reflux extraction time,extraction times,and solid/liquid ratio,the content of glaucocalyxin A was determined by HPLC.Results：The conditions under which the extraction process was optimized is A2B2C3,each 1.5 hours,reflux extraction twice,S/L amount 1：15;the content of glaucocalyxin A was the highest.Conclusion：The method is simple,rapid and accurate.It can be used for determining the content of glaucocalyxin A in Rabdosia japonica var.glaucocalyx.
出处
《抗感染药学》
2010年第4期240-242,共3页
Anti-infection Pharmacy
基金
苏州市科技支撑计划(编号:SS0933)
苏州大学大学生课外学术科研基金项目(编号:Q3132823)
关键词
蓝萼香茶菜
蓝萼甲素
正交设计法
含质量测定
Rabdosia japonica var.glaucocalyx
glaucocalyxin A
orthogonal design
determination
