摘要
参照国内外已发表的猪传染性胃肠炎病毒(TGEV)和猪流行性腹泻病毒(PEDV)基因序列及其相关的RT-PCR检测方法,根据TGEVS蛋白基因和PEDVS基因各设计一套特异性通用引物,扩增目的带分别为426bp和584bp。通过对相关病毒检测,建立了TGEV和PEDV通用型二联RT-PCR检测方法。该方法具有快速、敏感、特异等优点,可为TGEV和PEDV的检测、流行病学调查及疫苗使用等奠定基础。
According to the published gene sequence of transmissible gastroenteritis virus of pigs（TGEV） and porcine epidemic diarrhea virus（PEDV） and the detecting methods of RT-PCR reported,matrix gene of TGEV and nucleoprotein（NP） gene of PEDV were considered as the target gene sequences of multiple RT-PCR.A set of universe primers were designed for detecting TGEV and PEDV,respectively in the conseved S gene of TGEV and S gene of PEDV.The products of RT-PCR is 426 bp and 584 bp respectively.The optimal condition of multiple RT-PCR for detection of TGEV and PEDV by RT-PCR was determined through orthogonal assay.The specificity of the multipie primers were examined by RT-PCR using template extracted from other avian virus.The sensitivity of RT-PCR were also determined by a seral dilution of RNA from TGEV and PEDV.This study had paved the way for testing TGEV and PEDV,defining pathogenic potential,using vaccines against TGEV and PEDV and so on.
出处
《中国农学通报》
CSCD
北大核心
2010年第23期26-29,共4页
Chinese Agricultural Science Bulletin
基金
辽宁医学院奥鸿药业大学生科技活动基金项目"猪传染性胃肠炎病毒和猪流行性腹泻病毒二联RT-PCR检测方法的建立"(2008D33)
