摘要
目的 观察甘氨酸在大鼠减体积肝移植中对移植后大鼠生存率、脏器损伤及肝脏再生的影响.方法 雌性Sprague-Dawley大鼠（200～230 g）分为甘氨酸组和对照组.甘氨酸组：术前经静脉给予供体甘氨酸1.5 ml（300 mmol/L）,对照组给予1.5 ml的林格氏液.50%大鼠肝脏在HTK液经过2 h的冷保存后行原位肝移植术.肝移植术后观察大鼠生存率、血清转氨酶、肝脏组织学及肝脏再生情况.结果大鼠减体积肝移植（50%）术后,甘氨酸显著延长大鼠生存率.减体积肝移植术后8 h对照组血清中的谷草转氨酶（AST）、谷丙转氨酶（ALT）及乳酸脱氢酶（LDH）分别升至（4045.4 ±614.5）、（1972.3±338.2）、（13 079.8±2608.4）U/L,而甘氨酸可降低血清转氨酶40%～50%（P〈0.05）.肝脏组织学也证明了甘氨酸的保护作用（P〈0.05）.移植术后8 h和24 h,肝脏的再生率和肝组织中的Ki-67表达在两组中差异有统计学意义.结论 甘氨酸在大鼠减体积肝移植术后可以延长生存率,减轻肝脏损伤,并且不阻碍肝脏的再生.Kuffer细胞的失活是其主要机制.
Objective To assess the effect of glycine on survival rate,liver damage and regeneration after rat size-reduced liver transplantation （50%）.Methods Female Sprague-Dawley rats （weighing 200-230 g） received glycine （ 1.5 ml,30 mmol/L） infusion before transplantation.Controls were given Ringer＇ s solution （ 1.5 ml）.Size-reduced liver transplantation （ 50% ） was performed after 2 h of cold storage.Animal survival,serum transaminases,liver histology,and liver regeneration were then investigated.Results After size-reduced liver transplantation （50%）,glycine significantly improved the survival rate as compared with controls.8 h after transplantation,the serum levels of aspartate aminotransferase （AST） ,alanine aminotransferase （ALT） and lactate dehydrogenase （LDH）in control group were increased to （4045.4 ± 614.5 ）,（ 1972.3 ± 338.2 ）,（ 13 079.8 ± 2608.4） U/L respectively.Those in glycine group were reduced by 40% to 50% of control group （P 〈 0.05 ）.The significant protective effects of glycine were also confirmed by the results of histology （P 〈0.05 ）.8 and 24 h after operation,there was no significant difference in hepatic regeneration rate and expression of Ki-67 between control and glycine groups.Conclusion These data suggest that glycine improves the survival and minimizes reperfusion injury,but doesn＇ t block the regeneration after size-reduced liver transplantation in vivo.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2010年第12期1850-1852,共3页
Chinese Journal of Experimental Surgery
关键词
肝移植
肝脏损伤
再生
甘氨酸
Live transplantation
Live injury
Regeneration
Glycine
