摘要
目的探讨干扰癌基因DJ-1表达对人乳腺癌耐药细胞株MCF-7/ADM多药耐药性(multi-drug resistence,MDR)的影响。方法将构建的DJ-1 shRNA真核表达载体,用脂质体转染至人乳腺癌耐药细胞株MCF-7/ADM;用RT-PCR和Western blot法分别检测细胞MDR 1 mRNA和P-糖蛋白(P-glycopro-tein,P-gp)的表达;MTT法检测细胞对化疗药物的敏感性。结果 DJ-1 shRNA靶向干预MCF-7/ADM细胞DJ-1表达后,DJ-1 shRNA组细胞MDR 1 mRNA和P-gp表达水平较空白与阴性对照组明显下降(P<0.0 1);细胞内Adriamycin浓度明显增加;细胞对Adriamycin的敏感性增强2.6 8倍。结论 DJ-1shRNA可降低乳腺癌MCF-7/ADM细胞的MDR,为研究逆转乳腺癌化疗耐药提供了新方法。
Objective To explore the effect of multidrug resistance（MDR） of human breast cancer Adriamycin resistant cell line MCF-7/ADM by interference gene DJ-1 expression.Methods DJ-1shRNA plasmid was transfected into MCF-7/ADM cells by lipofectamine 2000;The mRNA and protein expression level of MDR1 was detected by RT-PCR and Western blot,respectively.The cellular sensitivity to chemotherapeutics was measured by MTT.Results Compared with blank and negative control groups MDR1 mRNA and P-gp expression were reduced after MCF-7/ADM cells interfered by DJ-1 sh′RNA,（all P0.01）.The chemosensitivity assays revealed that the transfected cells sensitivity to chemotherapeutics adriamycin was increased 2.68 fold.Conclusions DJ-1shRNA effectively reverses the multidrug resistance of human breast cancer Adriamycin resistant cell line MCF-7/ADM,which provides a novel approach for reversing breast cancer MDR.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2010年第11期1219-1222,共4页
China Journal of General Surgery
基金
湖北省自然科学基金资助项目(2009CBB323)
华中科技大学研究生科技创新基金资助项目(HF-07-58-2010-540)
关键词
DJ-1
RNA干扰
多药耐药
乳腺癌
DJ-1
RNA interference
Multidrug resistance
Breast cancer
