摘要
目的观察大鼠在脑损伤后额叶皮层、海马、伏隔核乙酰胆碱转移酶(ChAT)和神经细胞黏附因子(NCAM)的变化,探讨ChAT、NCAM变化与脑损伤后认知障碍的关系。方法以自由落体打击建立轻型和中型脑损伤大鼠模型,以"假手术"大鼠的脑组织为对照。水迷宫检测伤后认知障碍变化,应用分光光度法测定额叶皮层、海马、伏隔核ChAT含量,应用ELISA法测定额叶皮层、海马、伏隔核NCAM含量。结果轻、中型脑损伤组大鼠于伤后2 d认知障碍最严重;伤后ChAT及NCAM含量均呈现先降后升的趋势,均于伤后2 d达到最低水平。结论脑损伤后认知障碍变化趋势同额叶皮层、海马、伏隔核ChAT和NCAM含量变化趋势有相似性。
Objective To investigate the relationship between the cognitive impairment and the expression of ChAT and NCAM in the cortex of frontal lobe,hippocampus and nucleus accumbens.Methods Based on Feeney＇s model of TBI,the brain tissue samples were taken at 1,2,3,5,7 d respectively after brain injury.The expression of ChAT and NCAM was examined by spectrophotometry and ELISA approach,and compared with sham-operation control group.Results The expression of ChAT and NCAM at 1 d after injury was lower than that of the sham-operation control group and reached the lowest level at 2 d.The test results of the learning and memory were adopted for the change.Conclusion The results suggest that the expression of ChAT and NCAM decreases following cognitive impairment,and ChAT and NCAM synthesized by the brain itself may play an important protective role in cognitive function of the brain.
出处
《福建医科大学学报》
2010年第5期320-323,共4页
Journal of Fujian Medical University
基金
福建省科技厅基金(2006Y0012)
福建医科大学教授学术发展基金(JS06035)
关键词
脑损伤
认知障碍
胆碱O-乙酰转移酶
神经细胞黏附分子类
brain injuries
cognition disorders
choline O-acetyltransferase
neural cell adhesion molecules
