摘要
从生姜根际土样分离获得的86个细菌分离物中,通过离体拮抗试验,筛选出2株对生姜茎腐病有良好拮抗效果的细菌,编号分别为JF24和JF3。通过对它们进行形态学观察、生理生化测定以及16S rRNA序列分析,初步确定,JF24为荧光假单胞菌(Pseudomonas fluorescens),JF3为粪产碱菌(Alcaligenes faecalis)。JF24和JF3的16S rRNA序列的GenBank登录号分别为FJ999730和FJ999731。
Eighty six bacterium isolates were obtained from rhizosphere of ginger.Through antagonistic tests,two antagonistic strains against Pythium myriotylum Drechsler were isolated,which were desig-nated as JF24 and JF3,respectively.Based on the morphological,physiological and biochemical char-acteristics and phylogenetic analysis of 16S rRNA,JF24 and JF3 were identified as Pseudomonas fluo-rescens and Alcaligenes faecalis,respectively.The sequences of 16S rRNA of them have been submit-ted to GenBank and the accession numbers are FJ999730 and FJ999731,respectively.
出处
《微生物学通报》
CAS
CSCD
北大核心
2010年第12期1767-1770,共4页
Microbiology China
基金
公益性行业科研专项经费项目资助(No.20090318)
关键词
生姜根际
茎腐病
拮抗
筛选
鉴定
Rhizosphere of ginger
Rhizome rot
Antagonistic
Screening
Identification
