摘要
目的：分析评价芩连四物汤及其各部位对小鼠离体子宫收缩模型的影响，并探讨物质基础与效应之间的关系。方法：采用大孔吸附树脂法分离制备各部位样品（QL-1-QL-14）；采用小鼠离体子宫收缩模型评价该方及各部位的生物效应；采用HPLC—DAD—ESI—MS法对活性显著部位QL-3中的主要色谱峰进行分析鉴定。结果：芩连四物汤拮抗小鼠离体子宫平滑肌收缩的主要活性部位是QL-1，QL-3，QL-5，QL-7，QL-115个部位；通过标准化合物对照及质谱特征对活性显著部位QL-3色谱峰进行了归属和指认，鉴定了其中29个化合物分别为苹果酸、没食子酸、梓醇、原儿茶酸、桃叶珊瑚苷、川芎嗪、香草酸、咖啡酸、芍药内酯苷、小檗红碱、芍药苷、四氢巴马汀、黄连碱、药根碱、益母草苷、甲基黄连碱、阿魏酸、巴马汀、小檗碱、野黄芩苷、黄芩素-7-O-葡萄糖苷、黄芩苷、地黄苷C、汉黄芩苷、白杨素-7—O-葡萄糖苷、千层纸苷、黄芩素、汉黄芩素、千层纸素-A。结论：芩连四物汤拮抗离体子宫收缩模型的主要活性部位为挥发油部位及去除大分子的醇沉上清液小分子部位；醇沉上清液小分子部位经大孔树脂吸附醇梯度洗脱得到了水洗脱部位，20％及60％醇洗脱3个活性部位。
Objective： To evaluate the bio-activity of Qinlian Siwu decoction on in vitro uterus contraction model and exploit the relationship between chemical components and the bio-activity. Method： The samples were prepared by maeroporous adsorptive resins. The in vitro uterus contraction model was adopted to appraise the bio-activities of Qinlian Siwu decoction and its different separated fractions. HPLC-DAD- ESI -MS method was applied to analyze and identify the components in the fraction QL-3. Result： It was found that five active fractions （ QL-1, QL-3, QL-5, QL-7 and QL-11 ） were separated from Qinlian Siwu decoction, mainly contributed to the observed antagonismto the contraction of the mouse uterus. 28 compounds in the fraction QL-3 were identified as malic acid, gallic acid, catalpol, protocatechuic acid, aucubin, ehuanxiongzine hydroehloridum, vanillie acid, caffeie acid, paeoniflofin, berberastine, albiflorin, tetrahydropalmatine, coptisine, jatrorrhizine, leonuride, worenine, ferulic acid, palmatine, berberine, scutellarin, baicalin-7-O-glucoside, baiealin, rehmannioside C, wogonoside, chrysin-7-glucuronide, ttetuin, baicalein, wogonin and oroxylin-A. Conclusion ： In vitro inhibiting the contraction of the isolated mouse uterine of Qinlian Siwu decoction was mainly attributed to the fraction QL-1 and QL-3. The active fractions （ QL-5, QL-7 and QL-11 ） were obtained from QL-3 on the maeroporous adsorptive resins by the gradient elution using ethanol
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第24期3362-3367,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30873235)
江苏省高校自然科学重大基础研究项目(06KJA36022)
江苏省自然科学基金项目(BK2008455)
国家教育部"新世纪优秀人才支持计划"项目(NCET-09-0163)
江苏高等学校优秀科技创新团队支持计划(2009年度)
南京中医药大学青年基金项目(09×ZR20)
