摘要
目的：建立维药刺糖中黄酮类化合物含量测定的荧光分析法。方法：根据黄酮类化合物的荧光性质,以芦丁为标准品,用荧光分析法测定了刺糖中黄酮类化合物的含量;并系统考察了溶剂、pH值、放置时间对荧光强度的影响。结果：在70%的乙醇中加入pH值7.0的三酸缓冲溶液,芦丁荧光强度最大,线性范围在2.7×10^-7-1.35×10^-5mol·L^-1,r=0.999 5,平均回收率为98.9%（RSD=1.9%,n=9）。结论：该方法准确度高、灵敏度高,适用于药用刺糖中黄酮类化合物的含量测定。
Objective：To establish fluorescence method for the determination of flavone contents in saccharum alhagi.Methods：According to fluorescent character of flavonoids,the flavone contents in saccharum alhagi were determined by spectrofluorimetry.Results：The fluorescence was the strongest while solvent was 70% ethanol with Britton-Robinson buffer（pH 7.0）.The linear concentration range of rutin was 2.7×10^-7-1.35×10^-5mol·L^-1 with a correlation coefficient of 0.9995.The average recovery of samples was 98.9%（n=9,RSD=1.9%）.Conclusion：The method is simple,rapid and accurate,which is suitable for determination of flavone contents in saccharum alhagi.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2010年第22期2103-2105,共3页
Chinese Journal of New Drugs
基金
新疆维吾尔自治区高校科研计划(XJEDU2009S49)
新疆医科大学创新基金(XJMEDU2007-14)
关键词
黄酮
荧光法
刺糖
fluorescence method
total flavone
saccharum alhagi
