摘要
目的:构建肝细胞生长因子cDNA真核表达载体。方法:提取大鼠胎肝总RNA,利用RT-PCR技术扩增出肝细胞生长因子cDNA,连接于载体pMD18-T质粒上,构建重组载体,经双酶切和DNA测序分析检测重组载体构建结果。结果:成功构建肝细胞生长因子cDNA真核表达载体。结论:为进一步研究HGF与肝癌的关系提供了实验基础。
Objective：To construct expression vector of HGF cDNA. Methods： We extracted the fetal liver total RNA, used RTPCR to amplify HGF cDNA, and then cloned them in pMD18 -T vector to construct recombinant vectors. The result of recombinant vector was identified using PCR and DNA sequencing. Results： HGF cDNA ex- pression vector was constructed successfully. Conclusion： Constructing HGF cDNA expression vector may provide an experimental basis for the further study of the relationship between HGF and hepatic carcinoma.
出处
《现代肿瘤医学》
CAS
2011年第1期1-3,共3页
Journal of Modern Oncology
基金
浙江省医院卫生科学研究基金项目(编号:2006A115)
浙江省绍兴市科技计划项目(编号:2007D10030)
关键词
肝细胞生长因子
质粒
表达载体
hepatocyte growth factor
plasmid
expression vector
