摘要
目的:观察α-硫辛酸在大鼠视网膜缺血再灌注损伤(retinal ischemia-reperfusion injury,RIRI)过程中对P53和Bax表达的影响。方法:将SD大鼠72只随机分成正常组、视网膜缺血再灌注组和α-硫辛酸干预组。同时后两组根据再灌注的时间不同分成6,24,48和72h组。通过前房灌注加压的方法制成RIRI动物模型,采用Western-blot与免疫组织化学法测定在大鼠RIRI模型中的P53和Bax表达的变化。结果:P53和Bax在正常视网膜组织中几乎不表达,在缺血灌注6h开始表达,24h达到高峰,48h开始下降,72h后表达已经明显减弱。α-硫辛酸干预组各观察指标变化趋势基本与缺血再灌注组相似,但表达明显减弱,两组间比较差异均有显著统计学意义(P<0.01)。结论:α-硫辛酸可抑制P53和Bax的表达,对大鼠RIRI有保护作用。
AIM：To investigate the effect of α-lipoic acid（α-LA） on the retinal morphology expression of P53 and Bax during retinal ischemia-reperfusion injury.
METHODS：A total of 72 SD rats were randomly divided into normal control group, ischemia- reperfusion model group and LA treatment group. the last two groups were subdivided in group 6 hours,24 hours,48 hours,72 hours after reperfusion. The models of retinal ischemia-reperfusion injury were made by elevating intraocular pressure. Immunohistochemistry and Western-blot were used to measure changes of P53 and Bax protein levels in retinal tissue.
RESULTS： No expression of P53 and Bax positive cells were found in normal group, at the ischemia-reperfusion groups.the expression of P53 and Bax began to increase at 6 hours after reperfusion, at 24 hours reached the peak, began to decrease at 48 hours,and weakened obviously at 72 hours . The therapy groups of α-lipoic acid have the same trend with the ischemic groups in each index, but the expressive intensity of each period weakened in evidence. There was significant difference （P〈0.01）.
CONCLUSION： P53 and Bax play an important role in the happening of retinal ischemia-reperfusion injury （RIRI）;α-LA can inhibit the expression of P53 and Bax cell apoptosis.
出处
《国际眼科杂志》
CAS
2011年第1期37-39,共3页
International Eye Science
基金
中国辽宁省教育厅科学技术研究基金资助项目(No.2008404)~~
