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骨良恶性肿瘤Bcl-2和p53蛋白表达及意义 被引量:4

Expression of Bcl-2 and p53 Oncoproteins in Benign and Malignant Bone Tumors
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摘要 【目的】探讨Bcl-2、p53在良恶性骨肿瘤组织中的蛋白表达情况及其生物学意义。【方法】应用ABC免疫组化方法对33例恶性骨肿瘤,包括19例骨肉瘤,14例软骨肉瘤和22例良性骨肿瘤(骨软骨瘤)组织中Bcl-2,p53的蛋白表达进行研究。【结果】恶性骨肿瘤组织中Bcl-2,p53蛋白的阳性表达率分别为51.5%和54.5%,其中骨肉瘤组织为63.2%和47.4%,软骨肉瘤组织为35.7%和64.3%;良性骨肿瘤组织阳性表达率为18.2%和22.7%。恶性骨肿瘤组织Bcl-2,p53蛋白阳性表达率明显高于良性骨肿瘤(P<0.05),骨肉瘤组织Bcl-2蛋白阳性表达率明显高于骨软骨瘤(P<0.01),软骨肉瘤组织p53蛋白阳性率明显高于骨软骨瘤(P<005)。AKP值增高组的恶性骨肿瘤组织p53蛋白阳性表达率(75%)明显高于AKP值正常值组(20%,P<0.05)。【结论】Bcl-2癌基因介导的凋亡紊乱和p53肿瘤抑制基因突变失活与恶性肿瘤的发生有关,Bcl-2蛋白表达活性可能与骨肿瘤的恶性程度有关,p53肿瘤抑制基因突变失活是恶性肿瘤细胞成骨活性增强的因素之一。标记羊抗鼠IgG和ABC试剂,购自Sigma公? To study the expression and biological significance of Bcl-2 and p53 oncoproteins in benign and malignant bone tumors. The expression of Bcl-2 and p53 oncoproteins were investigated in 33 cases of malignant bone tumors,including 19 cases of osteosarcoma and 14 cases of chondrosarcoma,and 22 cases of benign bone tumors (osteochondroma) by using Avidin-Biotin Complex (ABC) immunohistochemical method. The positive expression rates of Bcl-2 and p53 oncoproteins were 51.5% and 54.5% in malignant bone tumors (osteosarcoma:63.2% and 47.4%;chondrosarcoma: 35.7% and 64.3%),whereas those in benign bone tumors were 18.2% and 22.7% respectively.The positive expression rates of Bcl-2 and p53 oncoproteins were significantly higher in malignant bone tumors than those in benign ones(P<0.05).The expression rate of Bcl-2 oncoprotein in osteosarcomas showed a positive rate significantly higher than that in osteochondromas (P<0.01),while the positive expression rate of p53 oncoprotein in chondrosarcomas was significantly higher than that in osteochondromas(P<0.05).The positive expression rate of p53 oncoprotein in the malignant bone tumors with increased AKP activities was distinctly higher than that with normal alkaline phosphatase(AKP) activities (75% vs 20%;P<0.05).ConclusionsThe genesis of malignant bone tumors may be related to the apoptosis disorders induced by Bcl-2 oncogene,and to the mutational inactivation of p53 tumor suppression gene.The degree of malignancy in bone tumors may be related to the expression efficiency of Bcl-2 oncoproteins.The mutational inactivation of p53 tumor suppression gene is one of the factors that promotes the osteogenic activity in malignant tumor cells of bone.
出处 《湖南医学》 1999年第3期171-173,共3页 Hunan Medical Journal
基金 湖南省卫生厅资助
关键词 骨肿瘤 BCL-2 P53蛋白 bone neoplasms oncogene proteins protein p53
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  • 1刘国辉,杨述华,郜勇,郭晓东.凋亡相关基因Survivin mRNA在骨肉瘤中表达的测定及其意义[J].中华实验外科杂志,2004,21(7):863-865. 被引量:2
  • 2许凌峰,王远成,俞炜源.Bcl-2蛋白的研究进展[J].生物技术通讯,2005,16(4):429-431. 被引量:7
  • 3刘金洋,李康华,廖前德.骨肉瘤中Bcl-2、Fas蛋白表达的相关性研究[J].医学临床研究,2006,23(4):581-582. 被引量:3
  • 4高天,郭乔楠.人骨肉瘤中bc1_2的表达与凋亡指数的关系及其临床意义[J].诊断病理学杂志,2006,13(5):365-367. 被引量:4
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