摘要
为得到应用于软组织重建的新型可注射组织工程支架材料,将经交联合成的HA交联凝胶(XLHA),分别与降解性能不同的两种温敏型材料聚N-异丙基丙烯酰胺(PNIPAAm)凝胶和甲基纤维素(MC),在常温下共混合成XLHA-PNIPAAm与XLHA-MC可注射水凝胶,分析XLHA-PNIPAAm与XLHA-MC水凝胶的可注射性能、耐酶解性能、温敏性能和化学结构,并进行溶血试验与细胞毒性试验评价可注射凝胶的生物相容性。研究获得了XLHA-PNIPAAm的可注射水凝胶的制备条件,并证实了分别将不可降解材料PNIPAAm与可降解材料MC和HA共混均能有效延缓HA的降解,且PNIPAAm延缓HA降解的效果更为显著。辐照剂量为5 kGy,MBAAm/NIPAAm(M/M)=0.015,NIPAAm单体浓度为3%,制备的PNIPAAm与XLHA共混制备的XLHA-PNIPAAm可注射凝胶的耐酶解性能最佳。差示扫描量热仪(DSC)检测显示XLHA-PNIPAAm的温敏性较为稳定。两种复合凝胶的细胞毒性和溶血试验均合格,且XLHA-PNIPAAm水凝胶的生物相容性要好于XLHA-MC凝胶。
In order to get a sort of new scaffold material for soft tissue reconstruction,we prepared XLHA-PNIPAAm and XLHA-MC injectable hydrogels by blending crosslinked HA（XLHA） and two temperature-sensitive materials,poly（N-isopropylacrylamide）（PNIPAAm） and Methylcellulose（MC） with different degradability,respectively.The injectablility,enzymatic biodegradability,temperature-sensitivity,structure cytotoxicity and hemolysis of them were tested.The preparation formula of XLHA-PNIPAAm injectable hydrogel was obtained,and it was verified that adding non-degradable material PNIPAAm could postpone the degradation of HA more effectively than that of the degradable material MC.PNIPAAm,prepared with 5 kGy dose radiation,MBAAm /NIPAAm（M /M） = 0.015,monomer concentration = 3%,produced XLHA-PNIPAAm with the slowest enzymatic degradation rate.DSC（Differential Scanning Calorimetry） detection revealed that temperature-sensitivity of the XLHA-PNIPAAm was more stable than that of XLHA-MC.Two composite hydrogels were qualified in cytotoxicity and hemolysis tests,and the biocompatibility of XLHA-PNIPAAm hydrogel was better than that of XLHA-MC hydrogel.
出处
《中国生物医学工程学报》
CAS
CSCD
北大核心
2010年第6期901-908,共8页
Chinese Journal of Biomedical Engineering
基金
国家重点基础研究发展(973)计划(2009CB930000)
