摘要
以猪笼草(Nepenthes mirabilis)腋芽为试材,研究不同浓度无机盐离子、蔗糖以及BA和NAA对猪笼草不定芽增殖和生根的影响。结果表明:最佳增殖培养基为1/2 MS基础培养基附加30 g/L蔗糖、2.0 mg/L BA和0.2 mg/L NAA;生根培养基以1/4 MS基础培养基附加0.3 g/L活性炭、15 g/L蔗糖、1.5 mg/L IBA和0.5mg/L NAA为最佳,猪笼草生根效果最好,生根率达到92%。
The axillary buds of Nepenthes mirabilis were used as specimens.The effect of different concentration inorganic salt,sucrose,BA,IBA and NAA for tissue culture and rapid propagation were studied.The results showed that the 1/2MS medium with sucrose 30 g/L,BA 2.0 mg/L and NAA 0.2 mg/ L was the best for multiplication culture.The 1/4MS medium with sucrose 15 g/L,IBA 1.5 mg /L and NAA 0.5 mg/L was the best treatment for inducing roots from shoots,and the rootage ratio was 92%.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2010年第6期115-118,共4页
Journal of Hebei Agricultural University
基金
河北农业大学大学生科技创新活动基金(2010SM03)
关键词
猪笼草
离体快繁
增殖
生根
Nepenthes mirabilis
tissue culture
multiplication
root
