摘要
以食品中分离到的L.mXFL0605株为试验菌株,PCR扩增得到iap全基因。将iap连接到pET-28a(+)载体进行原核表达,分别采用培养温度为25℃或37℃,IPTG浓度为5μg/mL或10μg/mL优化组合进行诱导。结果显示,当采用37℃培养和10μg/mLIPTG诱导时可以获得部分可溶性表达的p60,重组p60蛋白分子质量约60 ku。
The iap gene encoding invasion associated protein（p60） could distinguish L.monocytogenes from Listeria for its interspecific difference.So the iap gene was an ideal diagnostic target for the development of detection systems for L.monocytogenes.The iap gene of XFL0605 strain isolated from food was cloned in a pET-28a（＋） vector for prokaryotic expression in Escherichia coli BL21.In order to obtain soluble expression of p60 protein,BL21 were induced by isopropyl-β-D-thiogalactopyranoside（IPTG） with 5 μg/mL or 10 μg/mL at the 25 ℃ or 37 ℃ respectively.The p60 about 60 ku was soluble at the condition of 37 ℃ and 10 μg/mL IPTG.
出处
《动物医学进展》
CSCD
北大核心
2010年第12期19-21,共3页
Progress In Veterinary Medicine
基金
中央高校基本科研业务费专项资金(5220410048)
