摘要
目的通过室内反选育,获得家蝇对残杀威敏感品系。依据家蝇敏感品系乙酰胆碱酯酶(AChE)敏感度建立不敏感AChE的区分剂量,同时对室外家蝇种群不敏感AChE进行检测,为延缓家蝇抗药性的检测提供依据。方法敏感品系选育采用反汰选法,采用生物化学方法对自然种群中AChE不敏感基因表现型进行检测。结果经过6次反汰选,原敏感品系家蝇对残杀威的敏感度提高到13.87倍。对北京市种群检测结果表明有40%以上个体具有不敏感AChE。结论家蝇敏感品系中含有不敏感AChE,需要继续反选育;自然种群为残杀威抗性种群,应停用残杀威,用其他类型的药剂替代,避免或延缓抗性发展。
Objective Propoxur-susceptible Musca domestica strains were established through reverse selective breeding in the laboratory setting. The discriminating concentration of insensitive acetylcholinesterase （AChE） was established according to the AChE sensitivity of the strains. The insensitive gene AChE phenotypes of outdoor M. domestica populations were investigated to provide the basis for delaying pesticide resistance development in M. domestica. Methods The susceptible strains were bred through elimination selection. The insensitive AChE phenotypes in the natural population were investigated by biochemical approaches. Results After 6 cycles of elimination, the sensitivity of the original susceptible strain of M. domestica to propoxur had a 13.87 fold increase. The investigation result showed that more than 40% of the natural population in Beijing had insensitive AChE. Conclusion Carrying insensitive AChE, the susceptible strain of M. domestica should be subject to further reverse selective breeding. The natural population was resistant to propoxur. Hence, the application of this pesticide should be stopped and replaced with an alternative to avoid or delay the development of pesticide resistance.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2010年第6期548-550,共3页
Chinese Journal of Vector Biology and Control
关键词
家蝇
乙酰胆碱酯酶
抗药性
残杀威
Musca domestica
Acetylcholinesterase
Insecticide resistance
Propoxur
