摘要
采用Sephadex-G50凝胶柱层析分离具有强抗氧化活性的小分子芸豆肽,以VC为对照,研究3种肽分离组分的还原能力及对羟自由基、超氧阴离子自由基和DPPH自由基的清除能力。结果表明:随着相对分子质量的减小和肽质量浓度的增加,其还原能力及对羟自由基、超氧阴离子自由基和DPPH自由基的清除能力逐渐增大;小分子芸豆肽(C组分)的还原能力低于VC;对羟自由基、超氧阴离子自由基和DPPH自由基的半清除质量浓度分别为2.301、2.553、1.386mg/mL。该小分子肽组分的相对分子质量主要集中在100～1000,该组分疏水性氨基酸含量为36.78%,其疏水值达到364.73kcal/mol。说明小分子肽组分的抗氧化活性与其氨基酸种类和组成、相对分子质量分布密切相关。
Kidney bean peptides（KBP） were fractionated by gel filtration on SephadexG-50.The antioxidant and free radicalscavenging activities of three KBP fractions（Fra-A,Fra-B,Fra-C） were investigated using reducing power and 1,1-diphenyl-2pycrylhydrazyl（DPPH）/superoxide/hydroxyl radical-scavenging assay.The reducing power activity of Fra-C（RP50 2.263 mg/mL） was lower than that of ascorbic acid（RP50 0.828 mg/mL）.Fra-C showed the strongest scavenging activity against DPPH,superoxideanion and hydroxyl radicals.The radical-scavenging effect was in a dose-dependent manner and the EC50 values for DPPH,superoxide and hydroxyl radicals were found to be 2.301,2.553 mg/mL and 1.386 mg/mL,respectively.The amino acid analysis showed that Fra-C had a hydrophobic amino acid content of 36.78% and a hydrophobicity of 364.73 kcal/mol.The molecular weight distribution of Fra-C was found to mainly vary from 100 to 1000.The antioxidant activity of Fra-C is clearly related to the amino acid composition,the content of THAA and the molecular mass.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第21期105-109,共5页
Food Science
关键词
芸豆肽
抗氧化活性
自由基清除能力
氨基酸组成
相对分子质量分布
kidney bean peptides（KBP）
antioxidant activity
free radical-scavenging activity
amino acid composition
molecular weight distribution
