摘要
为评估柔鱼皮明胶体外消化产物的抗氧化活性及其特征,采用体外胃肠消化模型制备柔鱼皮明胶水解物(SHGD),对其氨基酸组成、抗氧化活性(还原能力、抑制脂类自氧化和清除自由基)和分子质量分布特征进行分析,结果表明:柔鱼SHGD氨基酸组成中富含32.78%的抗氧化特征氨基酸(酪氨酸、组氨酸、脯氨酸、丙氨酸和亮氨酸),该组成中总疏水性氨基酸(THAA)含量(37.48%)也较未水解柔鱼明胶高;20mg/mL和25mg/mL的柔鱼SHGD具有同丁基羟基茴香醚(BHA)组相似程度的还原能力;柔鱼SHGD在质量浓度为16mg/mL和12mg/mL时,DPPH自由基清除效果分别优于BHA组和α-生育酚组;质量浓度为25mg/mL时的柔鱼SHGD,清除羟自由基和超氧自由基的效果分别为(81.93±2.02)%和(72.26±2.12)%,其抑制脂类过氧化效果相对BHA组也较强;相同质量浓度的柔鱼皮明胶抗氧化效果明显劣于柔鱼SHGD;另外,柔鱼SHGD中分子质量范围为383～1492u的组分可能与其抗氧化活性有关。
In order to evaluate the antioxidant activity and molecular weight distribution of in vitro digestion product of flying squid（Ommastrephes batramii） skin-gelatin,the preparation of flying squid skin-gelatin hydrolysate derived from in vitro gastrointestinal（GI） digestion（SHGD） was performed using a simulated model and SHGD was analyzed for its amino acid composition,antioxidant effects（reducing power,inhibitory effect on lipid autooxidation and free radical scavenging capacity） and molecular weight distribution.SHGD was rich in antioxidant amino acids including tyrosine,histidine,proline,alanine and leucine,and their total content was 32.78%.The content of total hydrophobic amino acids（THAA）（37.48%） was comparatively higher than that of skin-gelatin.With increasing concentration from 20 mg/mL to 25 mg/mL,the reducing power of SHGD presented no obvious change,and was similar to that of butylated hydroxyanisole（BHA）.At concentrations of 16 mg/mL and 12 mg/mL,SHGD showed better ability to scavenge DPPH free radicals than BHA and α-tocopherol,respectively.The scavenging rates of SHGD at 25 mg/mL against hydroxyl and superoxide anion free radicals were（81.93±2.02）% and（72.26± 2.12）%,respectively.Meanwhile,SHGD was a stronger inhibitor against lipid peroxidation when compared to BHA.At the same concentration conditions,flying squid skin-gelatin was remarkably inferior to SHGD in antioxidant effect.Furthermore,based on our experimental results,it is deduced that the fractions of SHGD having a molecular weight varying from 383 to 1492 u might be responsible for its antioxidant activity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第21期123-130,共8页
Food Science
基金
浙江省科技厅面上重点项目(2008C22052)
关键词
柔鱼
皮明胶
胃肠消化
抗氧化
水解物
flying squid
skin-gelatin
gastrointestinal digestion
antioxidant
hydrolysate
