摘要
建立水产品中苯丙酸诺龙、诺龙残留量的液相色谱-串联质谱(HPLC-MS/MS)检测方法。样品用混合溶剂提取,提取液经浓缩溶解后采用液液萃取和C18柱净化,液相色谱-串联质谱检测。结果表明:苯丙酸诺龙、诺龙在0.005～0.25μg/mL范围内线性关系良好,在3个浓度添加水平下,两者的回收率分别为84.2%～91.3%、76.8%～98.6%,相对标准偏差(RSD)分别为6.8%～13.6%、3.3%～11.3%,最低检测限为5.0μg/kg。
A method was established for the determination of nandrolone and nandrolone phenylpropionate residues in aquatic products. Samples were extracted with mixed solution after enzymatic hydrolysis, purified with liquid-liquid extraction. Then the impurities were removed by C18 column, and the eluant was concentrated and subjected to liquid chromatography-tamden mass spectrometry analysis. Good linear correlation was obtained between the peak area and the concentration in the range of 0.005 0.25μg/mL. The spike recoveries were 76.8%-98.6% and 84.2%-91.3% for nandrolone and nandrolone phenylpropionate standard. The relative standard deviations were all below 13.6%, and the limits of detection was 5.0 μ g/kg.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第22期394-397,共4页
Food Science
基金
中国水产科学院东海水产研究所中央级公益型科研院所基本科研专项(2007T03)
关键词
液相色谱-串联质谱
苯丙酸诺龙
残留量
水产品
liquid chromatography-tandem mass spectrometry
nandrolone phenylpropionate
residue
aquatic products
