摘要
目的为了寻找新的肿瘤细胞分化诱导剂，探讨蝌蚪醚提取液（Ｔ８７１２）的抗肿瘤作用。方法将ＨＬ６０细胞培养于Ｔ８７１２和ＲＰＭＩ１６４０按１：２００（ｖ／ｖ）混合的培养基中。结果Ｔ８７１２能诱导ＨＬ６０细胞向单核／巨噬细胞方向分化，表现为生长抑制，ＮＢＴ还原能力提高，酸性非特异性酯酶（ＡＮＡＥ）活性增加，形态学观察类似单核／巨噬细胞。当Ｔ８７１２与ＲＰＭＩ１６４０培养基按１：１００（ｖ／ｖ）混合则可诱导ＨＬ６０细胞凋亡，如出现凋亡的形态学特征（凋亡小体出现等）；ＤＮＡ琼脂糖凝胶电泳显示典型的阶梯型带；流式细胞式分析证明凋亡细胞数增加，但Ｂｃｌ－２蛋白未有明显改变。结论Ｔ８７１２能抑制ＨＬ６０细胞增殖，在不同浓度条件下可诱导ＨＬ６０细胞向单核／巨噬样细胞分化或发生凋亡，其凋亡过程可能与Ｂｃｌ－２无明显相关性。
Objective\ To investigate the effect of experimentally induced cryptorchidism on apoptosis in testes of adult mice and the distribution changes of Bcl 2, Bax protein. Methods\ Total DNA was isolated from testes and was electrophoretically separated in 1 5% agarose gel, TUNEL method, biotin avidin DCS system indirect immunofluorescence techniques were used. Results\ DNA fragmentation, increasing apoptotic germ cells, in coordination with the loss of testicular weight, were observed on 6 15d in the cryptorchid testes. By TUNEL staining it was noted that postive cells were most frequently among primary spermatocytes and round spermatids. Bcl 2, Bax protein mainly distributed in cytoplasm of germ cells. With the increase of apoptosis, Bcl 2 was down and Bax was up regulated. Conclusion\ Temperature, Bcl 2, and Bax protein play an important role in male germ cell apoptosis.
出处
《解剖学报》
CAS
CSCD
北大核心
1999年第1期78-81,I014,共5页
Acta Anatomica Sinica
