摘要
目的观察庆大霉素对兔成骨细胞增殖及超微结构的影响。方法从出生２周的新西兰兔颅盖骨获取成骨细胞，在含不同浓度庆大霉素的ＤＭＥＭ培养液中培养２４小时或７２小时，采用四唑盐比色法观察庆大霉素对成骨细胞增殖的影响，并对在含不同浓度庆大霉素的ＤＭＥＭ培养液中培养９６小时的成骨细胞进行透射电镜观察。结果高浓度庆大霉素组（８００ｇ／ｍｌ、１６００ｇ／ｍｌ）光吸收值明显低于对照组（Ｐ＜０．０１），高浓度庆大霉素组超微结构表现为内质网扩张，胞浆内见大量的吞噬溶酶体。结论高浓度庆大霉素明显抑制成骨细胞增殖，呈现一定的毒性作用。
Objective To observe the effects of gentamycin on the proliferation and ultrastructure ofprimary cultured rabbit osteoblasts. Methods Osteoblasts isolated from the two-week newly born rabbits werecultured for 24 h or 72 h in DMEM media with different concentrations of gentamycin- MTT method was used toobserve the effect of gentamycin on cell proliferation. The ultrastructural changes of cultured osteoblasts exposedto gentamycin for 96 h were also observed under transmission electron microscope. Results The opticaldensity values of higher concentration gentamycin groups (800 g/ml, 1600 g/ml) were much smaller thanthat of the control group ( P < 0. 01 ) . The swelling of mitochondria and the presence of abundant phagolyso-somes were characterized as the ultrastructural features. Conclusion High concentration gentamycindemonstrated a certain toxic inhibitive effects to osteoblasts.[
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1999年第3期177-179,I003,共4页
Chinese Journal of Orthopaedics
基金
军队九五指令性课题基金
