摘要
目的评价骨癌痛大鼠脊髓NF，κB、IL-6和TNF-α表达的变化。方法雌性健康SD大鼠72只，体重150～180g，随机分为3组（n=24）：对照组（C组）、假手术组（S组）和骨癌痛组（BP组）。BP组于大鼠左侧胫骨干骺端骨髓腔内注射5μl Walker 256（1×10^5）乳腺癌细胞制备骨癌痛模型，S组左胫骨上端注射Hank液5μl。分别于肿瘤细胞接种前、接种后2、4、6、9、12、14、16、18和21d时测定机械阈值。分别于肿瘤细胞接种后6、12和18d时，处死3只大鼠，取L4-6脊髓组织，采用RT-PCR法测定NF—KBp65mRNA、IL-6mRNA和TNF—dmRNA的表达；另处死3只大鼠，取L4-6。脊髓组织，计数NF-κB阳性细胞，采用免疫组化法测定NF-κB p65表达。结果与C组和S组比较，BP组机械痛阈降低，脊髓NF—κB p65及其mRNA、IL-6mRNA和TNF—α mRNA的表达上调，NF—κB p65阳性细胞计数增加（P〈0．05或0．01）；C组和s组间上述指标比较差异无统计学意义（P〉0．05）。结论大鼠胫骨骨髓腔内注射肿瘤细胞通过激活脊髓NF-κB，导致炎性细胞因子IL-6和TNF-α大量释放，从而诱发骨癌痛。
Objective To evaluate the changes in expression of NF-κB, IL-6 and TNF-α in spinal cord in a rat model of bone cancer.Methods Seventy-two female SD rats weighing 150-180 g were randomly divided into 3 groups （n = 24 each） ： control group （group C） ; sham operation group （group S） and bone cancer pain group （group BP） . Bone cancer was induced by intra-tibial inoculation of 1 x 105 Walker 256 breast cancer cells. Paw withdrawal threshold to mechanical stimulation was measured with yon Frey filaments. The expression of NF-κB p65, IL-6 and TNF-α mRNA in the spinal cord was determined by RT-PCR and the expression of NF-κB p65 by immuno-histoehemistry and NF-κB p65 positive cell count was determined. Results The paw withdrawal threshold was significantly lower and the expression of NF-κB p65, NF-κB p65 mRNA, IL-6 mRNA, TNF-α mRNA and NF-κB p65 positive cell count in the spinal cord were significantly higher in group BP than in group C and S （ P 〈 0.05 or 0.01 ）. Conclusion Intra-tibial inoculation of Walker 256 breast cancer cells activates NF-κB in the spinal cord, leading to the increased release of IL-6 and TNF-α and mechanical hyperalgesia.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第9期1030-1033,共4页
Chinese Journal of Anesthesiology
基金
国家自然科学基金（30872442）
国家青年科学基金（H0903-81000479）
江苏省卫生厅基金（H200855）
江苏省卫生厅重点基金（K200518）
