摘要
柏树火焰层孔菌是中国以及亚洲菌物的新记录属中的新记录种,是一种稀有的药用菌。为寻求高产的生长条件和经济的培养基,进行转速、温度、pH值、培养液装瓶体积、接种量、碳源、氮源、C/N对菌丝体生长影响的单因素试验和多因素试验设计,利用液体培养基探索这些条件对菌丝体产量的影响。结果表明:菌丝生长最适碳源为淀粉,最适氮源为麸皮,P、K、Mg对菌丝的生长具有促进作用,最适C/N为20:1～25:1;菌丝生长的最适转速为150r/min,温度为25～30℃,最佳pH值为5～7,最适培养液装瓶体积为100mL,最适接种量为8mL/100mL。液体培养基的最佳营养条件为:葡萄糖25g/L、麸皮125g/L、K2HPO40.25g/L、pH6.5。通过生长曲线实验得到Logsitic方程为:y=0.22/(1+1.019e-0.013x)。
Pyrofomes demidoffii is a newly recorded species of a newly recorded genus in China, and it is also a rare medicinal mushroom. To find a both high yield and economical medium for the mycelial growth of Pyrofomes demidoffii, several culture conditions including carbon source, nitrogen source, C/N ratio, rational speed, culture temperature, initial medium pH value, the volume of medium in the fermentation vessel and inoculum amount were investigated through single factor experiments and the effects of three medium components and pH on the mycelial growth of Pyrofomes demidoffii were analyzed by multifactor uniform design. The above investigations were performed using a liquid medium. The optimum carbon source, nitrogen source and C/N ratio for the mycelial growth of Pyrofomes demidoffii were starch, wheat bran and 20：1 -- 25：1. The mycelial growth of Pyrofomes demidoffii was promoted by P, K and Mg. A pH range between 5 and 7, the volume of medium in the fermentation vessel of 100 mL and inoculum amount of 8 mL/100mL were found optimum. The optimum composition of the liquid medium used consisted of glucose 25 g/L, bran 125 g/L and K2HPO4 0.25 g/L, pH 6.5, The mycelial growth of Pyrofomes demidoffii could be described by the logistic equation： y = 0.22/（1＋ 1.019e - 0.013x）.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第23期146-150,共5页
Food Science
基金
四川省科技厅平台建设项目(08zh0009)
西南科技大学研究生创新基金项目(09ycjj02)
关键词
柏树火焰层孔菌
液体发酵
菌丝体产量
Pyrofomes demidoffii
liquid-state fermentation
mycelial yield
