摘要
目的评价γ-干扰素释放分析结核感染T细胞斑点试验(T-SPOT.TB)检测在结核性疾病中的诊断价值。方法对仁济医院疑诊或待排结核患者进行T-SPOT.TB检测。结果 T-SPOT.TB检测在结核性疾病阳性检出率为94.4%(17/18),明显高于PPD的47.1%(8/17)、结核抗体检查的12.5%(2/16)、结核PCR的16.7%(2/12)、抗酸杆菌涂片的7.7%(1/13)、结核菌培养的27.3%(3/11),差异有统计学意义(P<0.05)。T-SPOT.TB检测诊断结核性疾病敏感性、特异性分别为94.4%、95.2%,显著优于PPD。结论 T-SPOT.TB检测是诊断结核的快速敏感方法,在结核性疾病诊断中有重要价值。
Objective To evaluate the diagnostic value of an interferon-gamma release assay T- SPOT. TB in tuberculosis. Methods The patients with suspected tuberculosis in Renji Hospital received T- SPOT. TB assay in the peripheral blood mononuclear cells（PBMCs）. Results T-SPOT. TB assay, PPD, anti- TB antibody test, M tuberculosis polymerasc chain reaction, acid-fast bacilli stain and cultures for M tuberculosis were positive in 94. 4% （17/18）, 47. 1% （8/17） , 12. 5% （2/16） , 16. 7% （2/12）, 7. 7% （ 1/ 13 ） and 27.3% （ 3/11 ）, respectively, of patients with active tuberculosis. The sensitivity and specificity of the T-SPOT. TB were 94. 4% （P 〈0. 05 between PPD and T-SPOT. TB） and 95.2% （P 〈0. 05 between PPD and T-SPOT. TB）. Conclusions T-SPOT. TB assay is rapid and high-sensitive tests for diagnosing tuberculosis.
出处
《中华临床医师杂志（电子版）》
CAS
2010年第12期64-67,共4页
Chinese Journal of Clinicians(Electronic Edition)
基金
上海市公共卫生优秀学科带头人项目(08GWD16)
