摘要
目的研究端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)在胃腺癌组织和胃良性病变组织中的表达特征,探讨hTERT与胃腺癌生物学行为的关系。方法采用免疫组化的方法,检测45例胃腺癌、14例胃溃疡、16例慢性胃炎及11例正常对照者胃镜活检标本中hTERT的表达,并通过IPP软件对图片进行图像分析,结合随访获得的临床资料进行统计学分析。结果 hTERT在正常胃黏膜、慢性胃炎、胃溃疡组织中表达的IOD值分别为(119.44±54.12),(159.94±78.67),(266.55±81.84),三组之间无显著差异(P>0.05),但正常胃黏膜与胃腺癌[早期:(3129.88±634.34),进展期:(5288.45±1303.64)]差异有显著性(P<0.01)。hTERT的表达程度与胃腺癌的分化程度、浸润深度、淋巴结转移、远处转移、临床分期及病理类型有密切关系(P<0.05),与年龄、性别及肿瘤部位无明显关系(P>0.05)。结论hTERT与胃腺癌的发生、转移、预后有重要关系。
【Objective】To investigate the relationship between human telomerase reverse transcriptase（hTERT） expression with carcinogenesis and metastasis of gastric adenocarcinoma.【Methods】Immunohistochemistry was used to detect human telomerase reverse transcriptase expression in 45 gastric adenocarcinoma tissue samples,14 gastric ulcer tissue samples,16 chronic gastritis tissue samples and 11 normal gastric mucosa tissue samples.Image Pro Plus was used as the method of quantitative image analysis by measuring integrated optical density（IOD）.【Results】No significant difference was observed between the expressions of hTERT in normal gastric mucosa group （119.44±54.12）,chronic gastritis group（159.94±78.67） and gastric ulcer group（266.55±81.84）（P 0.05）.However,the expressions of hTERT in gastric adenocarcinoma groups [early stage：（3129.88±634.34）,and progressive stage：（5288.45±1303.64）] were significantly higher than that of the normal gastric mucosa group（P 0.01）.Close relationships between hTERT expression IOD and degree of differentiation,depth of tumor,clinical stage,lymph nodemetastasis,distant metastasis and pathological types were observed in gastric adenocarcinomas（P 0.05）,while no relationships between hTERT expression IOD and age,gender and tumor location were observed（P 0.05）.【Conclusion】Overexpression of hTERT is involved in carcinogenesis,metastasis and prognosis of gastric adenocarcinoma.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2010年第21期3201-3204,共4页
China Journal of Modern Medicine
