摘要
建立HPLC-DAD法测定肠循环液中的间尼索地平,探讨S型和R型间尼索地平在大鼠各肠段的吸收动力学特征,考察药物浓度、pH和吐温-80浓度对药物肠吸收的影响。采用大鼠在体小肠回流装置,使用C_(18)色谱柱,以乙腈-1.0%磷酸(62∶38)为流动相,检测波长353 nm。结果显示间尼索地平对映体在1～80μg/ml浓度范围内与吸收量的线性关系良好,吸收速率常数K_a基本保持不变;各肠段的吸收速率无显著性差异。全肠段吸收呈现一级动力学过程,吸收机制为被动扩散。
An HPLC-DAD method was established for the determination of m-nisoldipine enantiomers in the circulation solution of intestine.The absorption kinetics of m-nisoldipine enantiomers at different intestine segments of rats and the influence of the drug concentration,pH and Tween-80 on the absorption kinetics were also investigated.The intestine in rats was cannulated for in situ recirculation.A C_（18） column was used with the mobile phase of acetonitrile- 1.0%phosphoric acid（62：38） at the detection wavelength of 353 nm.The calibration curve of m-nisoldipine enantiomers was linear in the concentration range of 1 - 80μg/ml.The absorption of m-nisoldipine enantiomers in rat＇s intestine was a first-order process with the passive diffusion mechanism.m-Nisoldipine enantiomers can be absorbed in whole intestinal segments.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2010年第12期918-921,共4页
Chinese Journal of Pharmaceuticals
基金
国家自然科学基金项目(30840098)
河北省自然科学基金资助项目(C2008001073)
