摘要
实验以茜素红(ARS)为荷移试剂,采用分光光度法研究了ARS与头孢哌酮(CPZ)形成络合物的光谱性质及其反应的适宜条件。实验结果表明:头孢哌酮在2.0～200μg/mL范围内遵循比尔定律,回归方程为:A=0.0036ρ+0.000095(:ρμg/mL),检出限0.75μg/mL,表观摩尔吸光系数2.64×104L·mol-1·cm-1,相关系数为r=0.9965,并初步探讨了反应机理。用实验方法对尿样及生物样品进行加标回收测定,回收率在95.4%～104.8%。
The visible spectral characteristics and the reaction conditions of complex of cefoperazone（CPZ） and alizarin red S（ARS） were investigated by spectrophotometry with ARS as a chromogenic reagent.The results showed that Beer＇s law was obeyed in the concentration range of 2.0～200 μg/mL for CPZ and the regression equation was A=0.0036ρ＋0.000095（ρ：μg/mL） with the correlation coefficient R of 0.9965,the apparent molar absorptivity was 2.64×104 L·mol-1·cm-1 and the detection limit was 0.75 μg/mL.Furthermore,the reaction mechanism was also explored elementarily.The proposed method has been successfully applied to the determination of CPZ in urine and biological samples,and the results were satisfying.
出处
《分析试验室》
CAS
CSCD
北大核心
2011年第1期48-50,共3页
Chinese Journal of Analysis Laboratory
基金
内蒙古教育厅基金项目(2009MS0206)资助
关键词
茜素红
头孢哌酮
分光光度法
Alizarin Red S
Cefoperazone
Spectrophotometry
