摘要
目的：通过正交实验探讨治疗糖尿病地知复方的配伍规律。方法：采用一次性腹腔注射链脲佐菌素（STZ）造成糖尿病小鼠模型。选用L9（3^4）正交表，进行实验分组。测定给药前后血糖，血清及肾组织的一氧化氮（NO）、丙二醛（MDA）含量。结果：根据正交实验结果分析得出，各味药降血糖作用大小依次为地黄→葛根→黄芪→知母，适宜的剂量水平为地黄3葛根2黄芪3知母3；降低血清NO水平作用强弱顺序为知母→黄芪→葛根→地黄，适宜剂量水平为知母3黄芪3葛根3地黄2；降低肾组织NO作用强度依次为地黄→知母→黄芪→葛根，适宜剂量水平为地黄2知母3黄芪1葛根2。降低血清MDA含量作用强弱为黄芪→葛根→地黄→知母，最佳剂量水平为黄芪3葛根2地黄2知母1；降低肾组织MDA作用强弱顺序为知母／地黄→黄芪→葛根，适宜的剂量水平为知母3地黄2黄芪3葛根3。结论：对氧化损伤的改善，综合起来主要是知母和地黄起作用。当把血糖和氧化应激指标综合考虑时，可以得出，在此方中药味地黄起主要作用，最佳剂量水平为地黄3知母3黄芪3葛根3。
Objective ： To study the compatibilities of the ingredients in formula Dizhi for diabetes treatment by orthogonal experiment. Method： Experimental diabetes was established by ip streptozocin （STZ） once in mice. The experimental groups were divided according to L9 （3^4） orthogonal array table. Blood glucose （before and after administration）, the contents of carbon monoxide （NO） and malondialdehyde （MDA） in serum and kidney were determined. Result： The results of orthogonal experiment showed that the order of hypoglycemic activity was Dihuang→Gegen→Huangqi→Zhimu with the optimal dosage of Dihuang3 Gegen2 Huangqi3 Zhimu3. The order of decreasing NO ability in serum was Zhimu→Huangqi→Gegen→Dihuang with the optimal dosage of Zhimu3 Huangqi3 Gegen3 Dihuang2 , and the order of decreasing the level of NO in kidney was Dihuang→Zhimu→Huangqi→Gegen with the optimal dosage of Dihuang2 Zhimu3 Huangqil Gegen2. The order of decreasing the level of MDA in serum was Huangqi→Gegen→Dihuang→Zhimu with the optimal dosage of Huangqi3 Gegen2 Dihuang2 Zhimu1 ,and the order of decreasing the level of MDA in kidney was Zhimu→Dihuang→ Huangqi→Gegen with the optimal dosage of Zhimu3 Dihuang2 Huangqi3 Gegen3. Conclusion： Herb Zhimu and Dihuang in the formula had the main effects on the improvement of oxidative stress, and Dihuang seemed as the most important herb in improving blood glucose and oxidative stress. The optimal dosage was Dihuang3 Zhimu3 Huangqi3 Gegen3.
出处
《中国药师》
CAS
2011年第1期37-40,共4页
China Pharmacist
