摘要
目的观察化疗前后结肠癌细胞株HT29、SW620中CD133^＋结肠癌细胞的数量变化，探讨化疗对CD133^＋结肠癌细胞的作用效果。方法取对数生长的结肠癌细胞株HT29、SW620，采用80mg／L5-氟尿嘧啶（5．Fu）和25mg／L奥沙利铂（Oxaliplatin）分别作用8h，并设对照组，利用CD133抗体进行标记后再用流式细胞仪进行检测，观察化疗前后CD133^＋细胞的比例。结果流式细胞仪检测结肠癌细胞株HT29，80mg／L5-Fu处理8h组中CD133^＋肿瘤细胞为45．00％，与未处理组（32．80％）比较结果差异有统计学意义（P〈0．01）；25mg／L奥沙利铂处理8h组中CD133^＋肿瘤细胞为55．30％，与未处理组和80mg／L5-Fu处理8h组比较结果差异有统计学意义（P〈0．01）。流式细胞仪检测结肠癌细胞株SW620，80mg／L5-Fu处理8h组中CD133^＋肿瘤细胞为47．10％，与未处理组（33．90％）比较结果差异有统计学意义（P〈0．01）；25mg／L奥沙利铂处理8h组中CD133。肿瘤细胞为56．50％，与未处理组和80mg／L5-Fu处理8h组比较结果差异有统计学意义（P〈0．01）。结论CD133^＋结肠癌细胞能明显抵抗化疗。
Objective To observe the changes of quantity of CD133 ＋ cells in HT29 and SW620 cell lines before and after chemotherapy, and detect the effect of chemotherapy on CD133 ＋ cells. Methods HT29 and SW620 cells in logarithmic growth phase were separately treated with 80 mg/L 5-fluorouracil （5-Fu） and 25 mg/L oxaliplatin for 8 h, and analyzed by flow cytometry after being marked by CD1339 antibody. The proportion of CD133 ＋ cells before and after chemotherapy was measured. Results For HT29 ceils, the proportion of CD133 ＋ cancer cells in the group treated with 80 mg/L 5-Fu for 8h was 45.00% with the difference being remarkable （ P 〈 0. 01 ） compared to the non-treatment group （ 32. 80% ）. The proportion of CD133 ＋ cancer cells in the group treated with 25 mg/L oxaliplatin for 8 h was 55.30% with the difference being remarkable （ P 〈 O. 01 ） compared with both the non-treatment group and the group treated with 80 mg/L 5-Fu for 8 h. For SW620 cells, the proportion of CD133 ＋ cancer cells in the group treated with 80 mg/L 5-Fu for 8 h was 47. 10% with the difference being remarkable （P 〈 0. 01 ） compared with the non-treatment group （33.90%）. The proportion of CD133 ＋ cancer cells in the group treated with 25 mg/L oxaliplatin for 8 h was 56. 50% with the difference being remarkable （ P 〈 0. 01 ） compared with both the non-treatment group and the group treated with 80 mg/L 5-Fu for 8 h. Conclusion CD133 positive colon cancer cells have anti-chemotherapy activity obviously.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第1期30-31,共2页
Chinese Journal of Experimental Surgery
