摘要
目的:观察中脑黑质多巴胺(DA)神经元细胞凋亡(Programmed Cell Death,PCD)的规律。方法:选用胚胎14d的胎鼠至生后3个月的大鼠,运用酪氨酸羟化酶(TH)抗体和TUNEL免疫组化双标染色和免疫电镜法。结果:DA神经元PCD的发生始于孕16(E16),但胚胎期处于较低水平且规律不一,可能由于神经元处于增殖、分化、迁移状态的缘故。生后PCD的发生呈双峰状,第一个高峰在P2,第二个高峰在P14,高峰期恰与神经元之间发生突触整合期相吻合。结论:认为中脑黑质DA神经元PCD的发生主要于生后的早期阶段,可能与其靶器官分泌的神经营养因子的调控作用有关。
Aim: To identify the time course of programmed cell death (PCD) during development of dopaminergic(DA) neurons in substantia nigra. Methods: We examined the DA neurons from E14 to P3M rat using tyrosine hyroxylase and TUNEL double immunostaining technique and immunoeleccron microscopy technique. Results: PCD in DA neurons first occurs at El6, yet the whole number during embryonic ages is low and there is no rule to grasp. These neurons may be in a state of proliferation, differentiation and migratation. PCD in postnatal DA neurons is biphasic. One peak begins at P2, the other occurs at P14. The period of maximal PCD just corresponds with synapse formation between neurons and their targets during development. Conclusion: PCD in DA neurons occurs mainly in early postnatal ages. It may be regulated by target-derived trophic fators.
出处
《中国临床神经科学》
1999年第2期66-69,共4页
Chinese Journal of Clinical Neurosciences
基金
本课题受"九五"全军医药卫生基金资助
