摘要
目的探索人脑胶质瘤细胞原代培养的方法,研究不同胶质瘤患者对抗肿瘤药物的敏感度。方法采用组织块培养法对39例人脑胶质瘤细胞进行原代培养,用MTT法检测胶质瘤细胞对阿霉素(ADM)、顺铂(DDP)、长春新碱(VCR)、威猛(teniposide,VM-26)和5-氟尿嘧啶(5-Fu)5种化疗药物的敏感度,并对其结果进行分析。结果脑胶质瘤细胞原代培养37例成功,2例失败,成功率94.9%;37例培养成功者药敏检测显示不同个体对不同抗肿瘤药物的抑制率存在明显差异,各药物的平均抑制率依次为VM-26>DDP>5-Fu>ADM>VCR,其敏感率分别为56.8%、51.4%、37.8%、24.3%和13.5%。结论体外胶质瘤细胞原代培养和MTT法检测化疗药物的敏感度是可行的,检测化疗药物敏感度对胶质瘤患者个体化的化疗具有一定价值。
Objective To establish the method of primary human glioma cells culture and to evaluate the sensitivity of different antitumor drugs on different glioma individuals.Methods The glioma cells from 39 glioma patients were cultured primarily by the tissue pieces culture method.To study the sensitivity of chemotherapeutic drugs of the glioma cells to five drugs,adriamycin（ADM）,cis-dichlorodiamine platinum（DDP）,vincristine （VCR）,teniposide（VM-26） and 5-Floxuridine（5-Fu） were tested by MTT assay,and the results were compared.Results The primary human glioma cells were successfully cultured in 37 samples and 2 samples failed,with a successful rate 94.9 %.The success in 37 cases showed that diversity of inhibitory rates of antitumor drugs was observed in samples from different individuals.The rank of sensitivity from high to low is：VM-26DDP5-FuADMVCR.The effective rate of VM-26、 DDP、5-Fu、ADM and VCR was 56.8%、51.4%、37.8%、24.3% and 13.5% respectively.Conclusion It is feasible to primary culture glioma cells and to test chemotherapeutic drug sensitivity by MTT method.Determination of in vitro drug sensitivity of glioma patients with chemotherapy for the individual has a certain value.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第12期1380-1382,共3页
Cancer Research on Prevention and Treatment
基金
河北省医学科学研究重点课题计划资助项目(07133)
河北省科学技术研究与发展指导计划资助项目(062761114)
