摘要
为明确浙江地区副猪嗜血杆菌(HPS)病的流行特点,提高对该病的免疫防控能力,本研究对HPS分离株进行生物学鉴定。结果表明,HPS在含有2%小牛血清和16μg/mL NAD的TSB培养基中生长良好,在TSB液体培养基中培养16 h～18 h后,活菌数达到峰值;6个分离株对小鼠的LD50分别为2.03×109、5.3×109、1.34×1010、7.0×109、1.17×1010和1.62×109;可在感染HPS豚鼠的心血中分离到该菌;ERIC-PCR指纹图谱分析结果表明,4个分离株与4型、5型和9型标准菌株图谱一致,但有2个分离株与15个血清型标准菌株图谱不吻合。研究提示浙江地区内HPS的散发、流行的原因可能是由于该细菌血清型复杂性造成的。
This paper reported the biological characteristics of 6 Haemophilus parasuis field isolates in Zhejiang area.The H.parasuis isolates were cultured in TSB medium containing 2% calf serum and 16 μg/mL NAD,and peak growth was detected after 16 h to 18 h culturing.The median lethal dose（LD50） of these isolates were 2.03×10^9,5.3×10^9,1.34×10^10,7.0×10^9,1.17×10^10,1.62×10^9,respectively.H.parasuis could be isolated from heart blood post experimental infection in guinea pigs.ERIC-PCR fingerprint analysis showed that the genomic fingerprints of these 4 isolates were homologous with 4,5 and 9 serotype,and the other 2 isolates were different from the 15 serovar reference stains.These results indicated that mutiple serotypes of H.parasuis existed in the fields,which could be the cause of H.parasuis outbreaks in Zhejiang area.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2010年第12期941-944,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
浙江地区科技计划项目(2007C12051)
浙江地区金华市科技计划项目(2007-2-041)
