摘要
目的探讨血红素氧合酶1基因启动子区(GT)n重复序列多态性与广东潮汕人群冠心病易感性的关系。方法采用荧光标记PCR和毛细管电泳相结合技术检测血红素氧合酶1基因启动子区(GT)n重复序列多态性在300例冠心病患者以及性别、年龄相匹配的182例对照者中等位基因和基因型频率分布差异,重复次数n≤25为S型等位基因,n>25为L型等位基因。Logistics回归分析基因型与吸烟、高血压、糖尿病、高血脂、家族史等冠心病危险因素间的交互作用。结果等位基因和基因型频率在冠心病组与对照组中分布差异无显著性,基因型与吸烟(OR为1.790,95%CI为1.110-2.886)、高血压(OR为1.552,95%CI为1.045-2.304)、糖尿病(OR为1.727,95%CI为1.018-2.928)交互作用增加个体冠心病患病风险,SL+LL基因型与吸烟作用最为显著(OR为2.517,95%CI为1.206-5.253)。结论血红素氧合酶1基因启动子区(GT)n重复序列多态性可作为潮汕人群伴危险因素个体冠心病易感风险评估的分子标记。
Aim To assess the association between the length of（GT）n repeats in the heme oxygenase-1（HO-1）gene promoter and the susceptibility for coronary heart disease（CHD）of Chaoshan population.Methods 300 patients with CHD and 182 control subjects with sex and age matching were collected.Amplification and labeling of PCR products for capillary electrophoresis were performed to genotype.（GT）n repeat polymorphism was classified into short（n≤25 for S allele）and long（n25 for L allele）alleles.Results Allelic and genotypic frequency did not differ significantly between CHD patients and control subjects.However,there were interaction between genotype and smoking（OR was 1.790,95%CI was 1.110-2.886）,hypertension（OR was 1.552,95%CI was 1.045-2.304）and diabetes（OR was 1.727,95%CI was 1.018-2.928）.Specially,the SL＋LL genotype had the highest risk（OR was 2.517,95%CI was 1.206-5.253）.Conclusion Our findings indicated that longer（GT）n repeat allele in HO-1 promoter is a susceptibility marker of CHD with risk factors in Chaoshan population.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2010年第10期807-809,共3页
Chinese Journal of Arteriosclerosis
基金
广东省自然科学基金资助(07008215)
关键词
冠心病
血红素氧合酶1
基因多态性
易感性
Coronary Heart Disease
Heme Oxygenase-1
Gene Polymorphism
Susceptibility
