摘要
Objective： To establish an experimental model of urethral stricture in rabbits. Methods： A total of 21 adult male New Zealand rabbits were included into group. After intravenous anesthesia, urethroscopy was performed with a pediatric resectoscope （F13）. Fifteen animals were randomly selected as the study group. A lcm-long circumferential electrocoagulation of the bulbar urethra was performed to these animals until ulceration of the mucosa. The remaining 6 animals underwent video urethroscopy without electrocoagulation, serving as controls. On the 30th day, retrograde urethrogram and urethroscopy were performed to evaluate urethral stricture formation, histological examinations （HE and Sirius Red staining） were done to assess urethral pathological change. Results： Two rabbits in study group died and no death occurred in controls. Based on urethrogram and urethroscopy, no rabbits in control group developed urethral stricture, while significant stricture formation was observed in every case of the study group. Histological examination showed a normal urethra in control cases, while at stricture site of the study group extensive fibrosis of muscle and submucous tis High collagen expression in fibrosis tissue was assayed sue by was observed with a large number of fibroblasts infiltration Sirius Red staining. Conclusion： A lcm-long endoscopic electrocoagulation can successfully induce urethral stricture formation in rabbit models. This method offers an ideal animal model for the fundamental and clinical study of urethral stricture
目的将在兔子建立尿道的苛评的一个试验性的模型。21 只成年雄的新西兰兔子全部的方法 A 被包括进组。在静脉内的麻醉以后， urethroscopy 与小儿科的 resectoscope (F13 ) 被执行。十五个动物随机作为学习组被选择。A 1cm-long 球根的尿道的环绕的电凝法被执行到这些动物直到 mucosa 的溃疡。留下没有电凝法， 6 个动物经历了录像 urethroscopy，用作控制。在第 30 天，后退 urethrogram 和 urethroscopy 被执行评估尿道的苛评形成，组织学的考试(他和染色的天狼星红) 被做估计尿道的病理学的变化。在学习的二只兔子组织的结果死了，没有死亡发生在控制。基于 urethrogram 和 urethroscopy，在控制的兔子都不组织发达尿道的苛评，当重要苛评形成在学习组的每种情况中被观察时。组织学的检查在控制情况中显示出一个正常尿道，当在学习组的苛评地点肌肉和 submucous 组织的广泛的纤维变性与很多成纤维细胞渗入被观察时。在纤维变性织物的高骨胶原表示是由染色的天狼星红的 assayed。内视镜的电凝法能成功地导致的结论 A 1cm-long 在兔子模型的尿道的苛评形成。这个方法为尿道的苛评的基本、临床的学习提供一个理想的动物模型。
基金
Supported by the Key Sci-Tech Research Project of Shaanxi Province(2006K12-G3)
